AgNOR status in Down's syndrome infants and a plausible phenotype formation hypothesis
| Autor: | Halil Demirtas |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Ribosomal Proteins
Genetics Gene Dosage Infant General Physics and Astronomy Ribosome biogenesis RNA Antigens Nuclear Cell Biology Ribosomal RNA Biology Ribosome Chromosomes RNA Ribosomal Structural Biology Ribosomal protein Humans General Materials Science Down Syndrome Nucleolus organizer region Chromosome 21 Gene |
| Zdroj: | Micron. 40:511-518 |
| ISSN: | 0968-4328 |
| DOI: | 10.1016/j.micron.2009.02.014 |
| Popis: | Down's syndrome (DS) or trisomy 21 is the most frequent genetic birth defect associated with mental retardation. Although DS has been known for more than a 100 years and its chromosomal basis recognized for half a century (1959), the underlying patho-mechanisms for the phenotype formation remain elusive and cannot be fully explained by simple gene dosage effect. The general consensus is that the extra chromosome 21 genes perturb the global metabolism of the body cells. Our experiments show that the most prominent metabolic perturbation occurs during ribosome biogenesis in the cells of DS babies/infants. In humans, ribosomal RNA (rRNA) gene families or nucleolar organizer regions (NORs) are localized at the secondary constriction (on the satellite stalks) of five pairs of acrocentric chromosomes (13, 14, 15, 21 and 22) and their activities are evaluated specifically either in metaphase or interphase through a procedure known as AgNOR or silver staining. Our successive AgNOR studies, supported by RNA and nuclear protein measurement, show that cells from DS infants produce more ribosomes than expected, accounting for the extra set of active rRNA gene family (1/6–1/11) situated on the extra chromosome 21. Thus, the presence of an extra chromosome 21 stimulates a global increase in ribosome biogenesis in cooperation with other NOR-bearing chromosomes, causing unnecessary rRNA and ribosomal proteins synthesis compared to controls. Following the description of NORs, AgNOR, AgNOR-proteins, AgNOR measurement and our experimental results, we propose that the extra RNA and protein synthesis can cause a fundamental handicap to DS infants, contributing to the formation of DS phenotypes, due to the wasted energy in producing unnecessary macromolecules, including energy (GTP)-dependent transport of the excessive ribosomes from the nucleus to the cytoplasm. |
| Databáze: | OpenAIRE |
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