YsxC, a Putative GTP-Binding Protein Essential for Growth of Bacillus subtilis 168
| Autor: | Colin R. Harwood, Zoltán Prágai |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
biology
G protein Mutant Gene Expression Mutagenesis (molecular biology technique) Genetics and Molecular Biology Bacillus subtilis biology.organism_classification Microbiology GTP-binding protein regulators Bacterial Proteins Biochemistry GTP-Binding Proteins Mutagenesis Essential gene Gene expression Molecular Biology Bacteria |
| Zdroj: | Journal of Bacteriology. 182:6819-6823 |
| ISSN: | 1098-5530 0021-9193 |
| DOI: | 10.1128/jb.182.23.6819-6823.2000 |
| Popis: | YsxC is a member of a family of GTP-binding proteins carried by a diverse range of organisms from bacteria to yeasts, plants, and humans. To resolve the issue of whether ysxC of Bacillus subtilis is essential for growth, we attempted to construct mutants in which ysxC was either inactivated or placed under the control of an inducible promoter. Viable mutants were obtained only in the latter case, and these were inducer dependent, demonstrating unambiguously that ysxC is an essential gene. |
| Databáze: | OpenAIRE |
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