Vascular smooth muscle cells on hyaluronic acid: culture and mechanical characterization of an engineered vascular construct
| Autor: | Sara Mantero, Elena Binda, Davide Camozzi, Marina Morigi, Maria Grazia Colombo, Barbara Imberti, Andrea Remuzzi |
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| Rok vydání: | 2004 |
| Předmět: |
Vascular smooth muscle
Cell Survival Swine Cell Culture Techniques Biocompatible Materials Extracellular matrix chemistry.chemical_compound Tissue engineering Blood vessel prosthesis Tensile Strength Materials Testing Hyaluronic acid Cell Adhesion medicine Animals Hyaluronic Acid Cells Cultured Cell Proliferation Cell Size Tissue Engineering biology Chemistry Cartilage General Engineering Endothelial Cells Settore ING-IND/34 - Bioingegneria Industriale Membranes Artificial Coronary Vessels Elasticity Blood Vessel Prosthesis Fibronectin medicine.anatomical_structure Cell culture biology.protein Biomedical engineering |
| Popis: | Esterified hyaluronic acid (HYAFF) is routinely used for clinical tissue-engineering applications such as skin and cartilage. The material is degraded by neotissue formation and degradation products are highly biocompatible. In the present article we investigate the possibility to culture vascular smooth muscle cells on this biodegradable material for the generation of tubular constructs to be used for vascular tissue engineering. We have evaluated cell attachment and growth, and the possibility to obtain a three-dimensional tubular shape culture from flat HYAFF sheets. We also evaluated the mechanical properties of the cell constructs, using a specific testing protocol, and compared them with the properties of segments of porcine coronary artery. Morphology and viability tests demonstrated that vascular cells, either from porcine or human origin, adhere and grow on nonwoven meshes of HYAFF, and that precoating of the material with fibronectin or collagen had a modest effect on cell growth and extracellular matrix production. Cell growth reached a maximum 7 days after seeding. Simple wrapping of flat sheets of nonwoven meshes containing vascular cells around a cylindrical mandrel, and culture under static conditions for 14 days, yielded tubular constructs suitable for mechanical tests. Despite cell colonization, constructs showed lower mechanical resistance as compared with porcine coronary arteries. The material used and the technique developed result in highly cellularized tubular constructs. Whether the mechanical properties may be improved by dynamic culture conditions is worthy of investigation. |
| Databáze: | OpenAIRE |
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