Determination of a HIV protease inhibitor (DMP 450) in animal and human plasma by solid-phase extraction and high-performance liquid chromatography
| Autor: | M. F. Grubb, C. L. Krauthauser, Linyee Shum, S. J. White, C. A. Robinson |
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| Rok vydání: | 1996 |
| Předmět: |
Acetonitriles
Pan troglodytes Potassium Compounds Sensitivity and Specificity High-performance liquid chromatography Phosphates chemistry.chemical_compound Dogs Potassium phosphate Animals Humans Urea Solid phase extraction Chromatography High Pressure Liquid Chromatography Elution Methanol Extraction (chemistry) Azepines HIV Protease Inhibitors Haplorhini General Chemistry Hydrogen-Ion Concentration Rats chemistry Ammonium acetate Quantitative analysis (chemistry) |
| Zdroj: | Journal of Chromatography B: Biomedical Sciences and Applications. 678:303-308 |
| ISSN: | 0378-4347 |
| Popis: | Extraction of DMP 450 from plasma was performed with C2 solid-phase extraction columns, using 0.1 M ammonium acetate in 90% methanol to elute DMP 450. The extraction recovery over the range of 10 to 10 000 ng/ml averaged 81.0, 96.2, 77.4, 95.2 and 68.0% from rat, dog, monkey, chimpanzee (25-10 000 ng/ml) and human plasma, respectively. HPLC analysis was carried out with a C18 column and a mobile phase of acetonitrile, methanol and 30 mM potassium phosphate (pH 3), the composition dependent on the type of plasma being analyzed, and monitored at a wavelength of 229 nm. Intra-day and inter-day coefficients of variation were less than 9.9 and 12.9%, respectively. Absolute differences were less than 11.5%. |
| Databáze: | OpenAIRE |
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