Investigation of the iron oxidation state in ovarian cancer using synchrotron micro X-ray absorption near edge structure - preliminary results

Autor: D. M. Krauze, Dariusz Adamek, M. Grzelak, Pawel Wrobel, Giulia Veronesi, Robert Jach, H. Castillo, Marek Lankosz, L. Chmura
Přispěvatelé: Faculty of Physics and Applied Computer Science [Kraków] (FPACS), AGH University of Science and Technology [Krakow, PL] (AGH UST), Laboratoire de Chimie et Biologie des Métaux (LCBM - UMR 5249), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), European Synchrotron Radiation Facility (ESRF), Department of Pathomorphology, Faculty of Medicine, Jagiellonian University Medical College, Department of Gynecology and Obstetrics, Faculty of Medicine, Jagiellonian University Medical College, Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Uniwersytet Jagielloński w Krakowie = Jagiellonian University (UJ)
Jazyk: angličtina
Rok vydání: 2018
Předmět:
Zdroj: Journal of Analytical Atomic Spectrometry
Journal of Analytical Atomic Spectrometry, Royal Society of Chemistry, 2018, 33 (10), pp.1638-1644. ⟨10.1039/C8JA00140E⟩
Journal of Analytical Atomic Spectrometry, 2018, 33 (10), pp.1638-1644. ⟨10.1039/C8JA00140E⟩
ISSN: 0267-9477
1364-5544
Popis: International audience; Iron as a trace element plays a role in regulating the homeostasis of the human body and therefore the concentration of iron in the body must be carefully monitored. Both deficiency and overload of iron could have a serious effect on health. Iron in excess can be carcinogenic in different ways. As a catalyst of Fenton's reaction it leads to the creation of reactive oxygen species which causes oxidative stress and DNA damage, among other things. Iron can also reduce the response of the immune system to cancer and induce increased cancer cell proliferation. Ovarian cancer is one of the most common neoplasms in women and has a low survival rate. For this reason, there is strong motivation to deepen the understanding of the processes that lead to the disease and take place in ovarian cancer tissue. In this study, cryo micro X-ray absorption near edge structure spectroscopy was used to analyse the oxidation state of iron in ovarian cancer samples. For calibration, organic and inorganic reference samples containing Fe in different oxidation states were used. To determine the trace element in the neoplasm samples, the pre-edge region, white line and absorption edge position of Fe K-edge XANES spectra were examined and compared to those of the recorded reference materials. A comparison of these XANES spectral features shows the existence of differences in the iron oxidation state between the neoplasm samples and suggests the possibility that a combination of iron ions can occur in one sample. Overall, further investigations are required to make a better comparison and determine what organic compounds are contained in the samples and possibly carry out a statistical analysis.
Databáze: OpenAIRE
Externí odkaz: