Identification of citrullinated eukaryotic translation initiation factor 4G1 as novel autoantigen in rheumatoid arthritis
Autor: | Yuko Okazaki, Akari Suzuki, Ryo Yamada, Miyako Ohtake-Yamanaka, Tetsufumi Inoue, Tetsuji Sawada, Terumitsu Hasebe, Kazuhiko Yamamoto |
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Rok vydání: | 2006 |
Předmět: |
Adult
Male Molecular Sequence Data Biophysics Autoimmunity Biology medicine.disease_cause Autoantigens Biochemistry Arthritis Rheumatoid chemistry.chemical_compound Eukaryotic translation Complementary DNA Citrulline medicine Humans Initiation factor Molecular Biology Aged Aged 80 and over Base Sequence Citrullination Cell Biology Middle Aged medicine.disease Molecular biology chemistry Rheumatoid arthritis Immunology PADI4 Female Eukaryotic Initiation Factor-4G |
Zdroj: | Biochemical and Biophysical Research Communications. 341:94-100 |
ISSN: | 0006-291X |
DOI: | 10.1016/j.bbrc.2005.12.160 |
Popis: | Antibodies against citrullinated proteins are highly specific for rheumatoid arthritis. We previously reported that functional variants of the gene encoding peptidylarginine deiminase type 4 were closely associated with RA. The purpose of this study was to investigate the citrullinated autoantigens recognized by serum samples from patients with RA. The human chondrocyte cDNA expression library was citrullinated by PADI4 and was immunoscreened with anti-modified citrulline antibodies and sera from patients with rheumatoid arthritis. One immunoreactive cDNA clone containing a 2480-base pair insert was isolated and sequence analysis revealed that the cDNA included a part of the eukaryotic translation initiation factor 4G1. Immunoreactivity against a recombinant citrullinated eIF4G1 fragment was observed with high specificity in 50.0% of RA patients. The levels of antibodies against citrullinated eIF4G1 were correlated with those of anti-CCP antibodies. Citrullinated eIF4G1 was identified as a candidate citrullinated autoantigen in RA patients. Citrullination of eIF4G1 may thus be involved in the pathogenesis of RA. |
Databáze: | OpenAIRE |
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