Oxidative stress and apoptotic effects of copper and cadmium in the zebrafish liver cell line ZFL
| Autor: | King Ming Chan, Man Long Kwok |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Health
Toxicology and Mutagenesis Cytotoxicity LC20 lethal concentration of 20 % population sod2 superoxide dismutase 2 gene 010501 environmental sciences Toxicology medicine.disease_cause 01 natural sciences Casp9 caspase 9 protein Cd cadmium 0302 clinical medicine LC lethal concentration Caspase Cu copper Caspase-9 biology Chemistry z zebrafish Liver cell Regular Article Cell biology Casp8 caspase 8 protein VE tocopherol (Vitamin E) GST glutathione-S-transferase protein Combined effects SOD superoxide dismutase proteins ccs copper chaperone for superoxide dismutase gene cat catalase gene PBS phosphate-buffered saline CAT catalase protein ybx1 Y box-binding protein 1 gene Caspase 3 Caspase 8 LC50 median lethal concentration 03 medical and health sciences Casp3 caspase 3 protein lcsh:RA1190-1270 gr glutathione reductase gene medicine ef1a elongation factor 1-alpha gene Cell damage 0105 earth and related environmental sciences ComputingMethodologies_COMPUTERGRAPHICS lcsh:Toxicology. Poisons NAC N-acetyl-l-cysteine sod1 superoxide dismutase 1 gene medicine.disease GR glutathione reductase protein gst glutathione-S-transferase gene mtDNA mitochondrial DNA Apoptosis BCS bathocuproinedisulfonic acid disodium salt biology.protein Mitochondrial function 030217 neurology & neurosurgery Oxidative stress |
| Zdroj: | Toxicology Reports, Vol 7, Iss, Pp 822-835 (2020) Toxicology Reports |
| ISSN: | 2214-7500 |
| Popis: | Graphical abstract Highlights • Oxidative stress and apoptosis created by Cu2+ and Cd2+ insults were studied in ZFL. • Cu2+ and Cd2+ both created lipid peroxidation, causing oxidative stress in cytoplasm. • Mitochondrial superoxide was induced by Cd2+ but supressed by Cu2+. • Cu2+ suppressed Casp3 activity, resulting in suppressed the apoptosis. • Pre-treatments of low concentration of Cu2+ protected the cell from Cd2+ insults. Copper (Cu) and cadmium (Cd) are widely used in industrial activities, resulting in Cu and Cd contamination in aquatic systems worldwide. Although Cu plays an essential role in many biological functions, an excessive amount of the metal causes cytotoxicity. In contrast, Cd is a non-essential metal that usually co-exists with Cu. Together, they cause oxidative stress in cells, leading to cell damage. These metal ions are also believed to cause cell apoptosis. In this study, we used a zebrafish liver cell line, ZFL, to study combined Cu and Cd cytotoxicity. Although Cd is more toxic than Cu, both were found to regulate the expression of oxidative stress related genes, and neither significantly altered the activity of oxidative stress related enzymes. Co-exposure tests with the antioxidant N-acetyl-l-cysteine and the Cu chelator bathocuproinedisulfonic acid disodium salt demonstrated that Cd toxicity was due to the oxidative stress caused by Cu, and that Cu at a low concentration could in fact exert an antioxidant effect against the oxidative stress in ZFL. Excessive Cu concentration triggered the expression of initiator caspases (caspase 8 and caspase 9) but suppressed that of an executioner caspase (caspase 3), halting apoptosis. Cd could only trigger the expression of initiator caspases; it could not halt apoptosis. However, a low concentration of Cu reduced the mitochondrial superoxide level, suppressing the Cd-induced apoptotic effects in ZFL. |
| Databáze: | OpenAIRE |
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