Replacing the decoy epitope of PCV2b capsid protein with a protective epitope enhances efficacy of PCV2b vaccine
| Autor: | Wenzhen Diao, Jiwei Liu, Liying Wang, Yaqin Yu, Hongfei Wei, Yongli Yu, Cheng Yu, Xin Li, Yue Xiao, Leichao Zhang |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Circovirus Swine 030106 microbiology Antibodies Viral environment and public health Epitope law.invention 03 medical and health sciences Mice Immune system law Animals Circoviridae Infections skin and connective tissue diseases Swine Diseases Vaccines Synthetic General Veterinary General Immunology and Microbiology Linear epitope biology Public Health Environmental and Occupational Health Viral Vaccines biology.organism_classification Virology biological factors Porcine circovirus 030104 developmental biology Infectious Diseases Treatment Outcome Capsid health occupations Recombinant DNA biology.protein Molecular Medicine Epitopes B-Lymphocyte Capsid Proteins Antibody Decoy |
| Zdroj: | Vaccine. 34(50) |
| ISSN: | 1873-2518 |
| Popis: | Microbial pathogens may evolve decoy epitopes to evade host immune responses. In recent years, a decoy epitope has been identified in the capsid protein (CP) of porcine circovirus type 2b (PCV2b) to divert the immune response away from protective epitopes in pigs. To avoid the decoy effect, we designed and produced a recombinant PCV2b CP (ΔCP) by replacing the decoy epitope with a neutralizing B cell epitope derived from CP and tested the ability of ΔCP to induce protective antibody responses in mice and pigs. As expected, the ΔCP, unlike inactivated PCV2b vaccines, recombinant PCV2b CP, and natural PCV2b infection, did not induce anti-decoy epitope antibodies. Although unable to form typical virus-like particles (VLPs), the ΔCP could increase the production of the anti-PCV2b antibodies among which no antibody against the decoy epitopes, and therefore induces improved protective immune responses in pigs challenged with PCV2b. These results provide an alternative strategy for development of recombinant subunit vaccines against PCV2b, and possibly other viruses, by replacing the decoy epitope with a protective epitope. |
| Databáze: | OpenAIRE |
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