Synthetic probes for in vitro purification and in vivo tracking of hepatocytes derived from human pluripotent stem cells
| Autor: | Choongseong Han, Hyo Sung Jung, Jong-Hoon Kim, Jong Seung Kim, Ji-Young Park, Gyunggyu Lee, Han-Jin Park, Gun Sik Cho, Jiyou Han, Hyojin Kim |
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| Rok vydání: | 2019 |
| Předmět: |
Indocyanine Green
Pluripotent Stem Cells Biophysics Cell Culture Techniques Bioengineering 02 engineering and technology Biomaterials Cell therapy 03 medical and health sciences chemistry.chemical_compound In vivo medicine Humans Induced pluripotent stem cell Cells Cultured 030304 developmental biology 0303 health sciences Cell sorting 021001 nanoscience & nanotechnology Flow Cytometry Imaging agent Cell biology Transplantation medicine.anatomical_structure chemistry Mechanics of Materials Hepatocyte Ceramics and Composites Hepatocytes 0210 nano-technology Indocyanine green |
| Zdroj: | Biomaterials. 222 |
| ISSN: | 1878-5905 |
| Popis: | Hepatocytes derived from human pluripotent stem cells (hPSCs) are promising candidates for cell therapy and drug discovery. However, it remains challenging to efficiently purify hepatocytes from undesired cell types after differentiation and to accurately monitor grafted cells after transplantation. Indocyanine Green (ICG), an FDA-approved, near-infrared (NIR) dye, has been used for various clinical purposes and is exclusively taken up by hepatocytes. However, ICG has a long emission wavelength (λem > 800 nm) that is beyond the detection range of fluorescence-activated cell sorting (FACS) systems. Moreover, it is easily eliminated from hepatocytes, hindering its application for NIR imaging. Here, we designed and synthesized two different probes based on the properties of ICG; 1) hepatocyte purifying agent (HPA, λem = 562 nm) for in vitro sorting and 2) hepatocyte imaging agent (HIA, λem = 817 nm) for efficient in vivo NIR imaging. We obtained highly enriched populations of hPSC-derived hepatocytes (hPSC-Heps) from various hPSC lines using HPA probe-based FACS purification. In addition, HIA labelling and NIR imaging allowed the direct visualization and tracking of grafted hPSC-Heps in animals with liver injuries. These results demonstrated that these two probes could be used as powerful tools with hPSC-Heps in both cell replacement therapy and drug screening. |
| Databáze: | OpenAIRE |
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