Neutrophil phenotype and function in partial hepatectomy in man
| Autor: | Katharina Holzer, Dirk Henrich, Dennis Hofmann, Elsie Oppermann, Christian Mönch, Wolf O. Bechstein, Stefan Zeuzem |
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| Rok vydání: | 2010 |
| Předmět: |
Adult
Male Neutrophils Phagocytosis Heterologous Receptors Cell Surface CD16 Andrology Downregulation and upregulation Hepatectomy Humans Medicine Receptor Opsonin Aged chemistry.chemical_classification Reactive oxygen species Cell adhesion molecule business.industry Liver Neoplasms Middle Aged Phenotype chemistry Immunology Female Surgery Reactive Oxygen Species business |
| Zdroj: | Langenbeck's Archives of Surgery. 395:643-653 |
| ISSN: | 1435-2451 1435-2443 |
| DOI: | 10.1007/s00423-009-0557-x |
| Popis: | Hepatic resections are still associated with considerable morbidity mainly because of postoperative infection. Adequate function of neutrophils is a crucial element in host defense. The aim of the study was to characterize neutrophils during partial hepatectomy. Fourteen patients undergoing partial liver resection were enrolled. Twenty-four hours pre-, intra- (after induction of anesthesia, after preparation of the liver, and 15 min after release of the Pringle maneuver), as well as postoperatively (3 h after Pringle; 24, 48, and 120 h after surgery), blood samples were obtained. In addition, healthy volunteers (n = 5) were investigated. Adhesion molecules (CD 62, CD 18), Fcy receptors (CD 16, CD 32), and phagocytosis by neutrophils were characterized by fluorescence-activated cell sorter analysis. Spontaneous and stimulated (formyl-methionyl-leucyl-phenylalanine) oxygen radical generation was measured by lucigenin-enhanced chemiluminescence. Numeric upregulation of CD 62 and CD 18 on neutrophils was seen before the use of Pringle maneuver and persisted thereafter (p |
| Databáze: | OpenAIRE |
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