Sulfation patterns of glycosaminoglycans encode molecular recognition and activity
Autor: | Manish Rawat, Nagarajan Vaidehi, Peter M. Clark, Cristal I. Gama, William A. Goddard, Naoki Sotogaku, Linda C. Hsieh-Wilson, Sarah E. Tully, Akinori Nishi |
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Rok vydání: | 2006 |
Předmět: |
Models
Molecular Cell Enlargement ENCODE Hippocampus Article Glycosaminoglycan Rats Sprague-Dawley chemistry.chemical_compound Structure-Activity Relationship Molecular recognition Sulfation Neurites Animals Chondroitin sulfate Molecular Biology Cells Cultured Glycosaminoglycans Molecular Structure Chemistry Sulfates Chondroitin Sulfates RNA Cell Biology Rats Biochemistry Function (biology) DNA |
Zdroj: | Nature chemical biology. 2(9) |
ISSN: | 1552-4450 |
Popis: | Although glycosaminoglycans contribute to diverse physiological processes, an understanding of their molecular mechanisms has been hampered by the inability to access homogeneous glycosaminoglycan structures. Here, we assembled well-defined chondroitin sulfate oligosaccharides using a convergent, synthetic approach that permits installation of sulfate groups at precise positions along the carbohydrate backbone. Using these defined structures, we demonstrate that specific sulfation motifs function as molecular recognition elements for growth factors and modulate neuronal growth. These results provide both fundamental insights into the role of sulfation and direct evidence for a 'sulfation code' whereby glycosaminoglycans encode functional information in a sequence-specific manner analogous to that of DNA, RNA and proteins. |
Databáze: | OpenAIRE |
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