A standardized gnotobiotic mouse model harboring a minimal 15-member mouse gut microbiota recapitulates SOPF/SPF phenotypes
| Autor: | Jennifer Yansouni, Filipe De Vadder, Martin Schwarzer, Dagmar Šrůtková, Ana Delgado, Alban Mathieu, Anne-Laure Bulteau, François Leulier, Andrei Bunescu, Céline Elie, Severine Planel, Bärbel Stecher, Lilia Boucinha, Djomangan Adama Ouattara, Céline Couturier, Andrea Tamellini, Tereza Novotná, Marion Darnaud, Adrien Saliou, Pascaline Bogeat, Helene Dugua |
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| Přispěvatelé: | Institut de Génomique Fonctionnelle de Lyon (IGFL), École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), BIOASTER Microbiology Technology Institute [Lyon], Institut de Génomique Fonctionnelle (IGF), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Institute of Microbiology of the Czech Academy of Sciences [Prague, Czech Republic] (MBU / CAS), Czech Academy of Sciences [Prague] (CAS), Ludwig-Maximilians University [Munich] (LMU), German Center for Infection Research, Partnersite Munich (DZIF), École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Leulier, François |
| Rok vydání: | 2019 |
| Předmět: |
Male
Physiology Science [SDV]Life Sciences [q-bio] General Physics and Astronomy Computational biology Gut flora digestive system General Biochemistry Genetics and Molecular Biology Article Feces 03 medical and health sciences 0302 clinical medicine Species Specificity [SDV.BA.ZV]Life Sciences [q-bio]/Animal biology/Vertebrate Zoology Animals Germ-Free Life [SDV.MP] Life Sciences [q-bio]/Microbiology and Parasitology 030304 developmental biology Genetics 2. Zero hunger 0303 health sciences Multidisciplinary Bacteria Whole Genome Sequencing biology 030302 biochemistry & molecular biology Body Weight Laboratory mouse Robustness (evolution) General Chemistry Fecal microbiota biology.organism_classification Confounding effect Phenotype Gut microbiome Gastrointestinal Microbiome Specific Pathogen-Free Organisms Mice Inbred C57BL [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology Metagenomics [SDV.BA.ZV] Life Sciences [q-bio]/Animal biology/Vertebrate Zoology Female Microbiome 030217 neurology & neurosurgery Microbiota composition |
| Zdroj: | Nature Communications Nature Communications, 2021, 12, pp.6686. ⟨10.1038/s41467-021-26963-9⟩ Nature Communications, Vol 12, Iss 1, Pp 1-21 (2021) |
| ISSN: | 2041-1723 |
| DOI: | 10.1101/2019.12.30.890954 |
| Popis: | Mus musculus is the classic mammalian model for biomedical research. Despite global efforts to standardize breeding and experimental procedures, the undefined composition and interindividual diversity of the microbiota of laboratory mice remains a limitation. In an attempt to standardize the gut microbiome in preclinical mouse studies, here we report the development of a simplified mouse microbiota composed of 15 strains from 7 of the 20 most prevalent bacterial families representative of the fecal microbiota of C57BL/6J Specific (and Opportunistic) Pathogen-Free (SPF/SOPF) animals and the derivation of a standardized gnotobiotic mouse model called GM15. GM15 recapitulates extensively the functionalities found in the C57BL/6J SOPF microbiota metagenome, and GM15 animals are phenotypically similar to SOPF or SPF animals in two different facilities. They are also less sensitive to the deleterious effects of post-weaning malnutrition. In this work, we show that the GM15 model provides increased reproducibility and robustness of preclinical studies by limiting the confounding effect of fluctuation in microbiota composition, and offers opportunities for research focused on how the microbiota shapes host physiology in health and disease. Here, the authors develop and characterize a mouse microbiota composed of 15 strains representative of the intestinal microbiota found in C57BL/6J specific opportunistic- and pathogen-free (C57Bl/6J SOPF) mice and derive a new standardized gnotobiotic mouse model, called GM15, which recapitulates the phenotypes of SOPF or SPF animals in different animal facilities with improved reproducibility. |
| Databáze: | OpenAIRE |
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