Applicability Evaluation of Male-Specific Coliphage-Based Detection Methods for Microbial Contamination Tracking
| Autor: | Kunbawui Park, Jina Ha, Min Cho, Minseok Kang, Gwoncheol Park, Seohyun Kang, Jiyoung Park, Gyungcheon Kim, Hakdong Shin, Sanghee Lee |
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| Rok vydání: | 2021 |
| Předmět: |
Oyster
Food Contamination Viral Plaque Assay Microbial contamination Coliphages Applied Microbiology and Biotechnology biology.animal medicine Animals Humans Seawater Coliphage Human virome Food science Dna viral Shellfish Food poisoning biology Reverse Transcriptase Polymerase Chain Reaction Virome food and beverages General Medicine biology.organism_classification medicine.disease DNA Viral Agar overlay RNA Viral Environmental Monitoring Biotechnology |
| Zdroj: | Journal of Microbiology and Biotechnology. 31:1709-1715 |
| ISSN: | 1738-8872 1017-7825 |
| DOI: | 10.4014/jmb.2110.10003 |
| Popis: | Outbreaks of food poisoning due to the consumption of norovirus-contaminated shellfish continue to occur. Male-specific (F+) coliphage has been suggested as an indicator of viral species due to the association with animal and human wastes. Here, we compared two methods, the double agar overlay and the quantitative real-time PCR (RT-PCR)-based method, for evaluating the applicability of F+ coliphage-based detection technique in microbial contamination tracking of shellfish samples. The RT-PCR-based method showed 1.6-39 times higher coliphage PFU values from spiked shellfish samples, in relation to the double agar overlay method. These differences indicated that the RT-PCR-based technique can detect both intact viruses and non-particle-protected viral DNA/RNA, suggesting that the RT-PCR based method could be a more efficient tool for tracking microbial contamination in shellfish. However, the virome information on F+ coliphage-contaminated oyster samples revealed that the high specificity of the RT-PCR- based method has a limitation in microbial contamination tracking due to the genomic diversity of F+ coliphages. Further research on the development of appropriate primer sets for microbial contamination tracking is therefore necessary. This study provides preliminary insight that should be examined in the search for suitable microbial contamination tracking methods to control the sanitation of shellfish and related seawater. |
| Databáze: | OpenAIRE |
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