Large-scale spontaneous self-organization and maturation of skeletal muscle tissues on ultra-compliant gelatin hydrogel substrates
| Autor: | Thomas E. Jensen, Joen H Jensen, Carmen Radeke, Martin Dufva, Johan Ulrik Lind, Christian Jonathan Pless, Morten Leth Jepsen, Selgin D Cakal, Jingwen Li |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
food.ingredient Muscle Fibers Skeletal lcsh:Medicine Gelatin Article Biomaterials Myoblasts 03 medical and health sciences Mice 0302 clinical medicine food Tissue engineering Myosin medicine Myocyte Animals lcsh:Science Muscle Skeletal Mechanical Phenomena Multidisciplinary Tissue Engineering Chemistry Myogenesis lcsh:R Skeletal muscle Hydrogels Biomechanical Phenomena Tissues 030104 developmental biology medicine.anatomical_structure Muscle contraction Self-healing hydrogels Musculoskeletal models Biophysics lcsh:Q Biomaterials - cells Gels and hydrogels C2C12 Biomedical engineering 030217 neurology & neurosurgery |
| Zdroj: | Jensen, J H, Cakal, S D, Li, J, Pless, C J, Radeke, C, Jepsen, M L, Jensen, T E, Dufva, M & Lind, J U 2020, ' Large-scale spontaneous self-organization and maturation of skeletal muscle tissues on ultra-compliant gelatin hydrogel substrates ', Scientific Reports, vol. 10, no. 1, 13305 . https://doi.org/10.1038/s41598-020-69936-6 Jensen, J H, Cakal, S D, Li, J, Pless, C J, Radeke, C, Jepsen, M L, Jensen, T E, Dufva, M & Lind, J U 2020, ' Large-scale spontaneous self-organization and maturation of skeletal muscle tissues on ultra-compliant gelatin hydrogel substrates ', Scientific Reports, vol. 10, 13305 . https://doi.org/10.1038/s41598-020-69936-6 Scientific Reports Scientific Reports, Vol 10, Iss 1, Pp 1-10 (2020) |
| DOI: | 10.1038/s41598-020-69936-6 |
| Popis: | Cellular self-organization is the fundamental driving force behind the complex architectures of native tissue. Yet, attempts at replicating native tissue architectures in vitro often involve complex micro-fabrication methods and materials. While impressive progress has been made within engineered models of striated muscle, the wide adaptation of these models is held back by the need for specific tools and knowhow. In this report, we show that C2C12 myoblasts spontaneously organize into highly aligned myotube tissues on the mm to cm scale, when cultured on sufficiently soft yet fully isotropic gelatin hydrogel substrates. Interestingly, we only observed this phenomenon for hydrogels with Young’s modulus of 6 kPa and below. For slightly more rigid compositions, only local micrometer-scale myotube organization was observed, similar to that seen in conventional polystyrene dishes. The hydrogel-supported myotubes could be cultured for multiple weeks and matured into highly contractile phenotypes with notable upregulation of myosin heavy chain, as compared to myotubes developed in conventional petri dishes. The procedure for casting the ultra-soft gelatin hydrogels is straight forward and compatible with standardized laboratory tools. It may thus serve as a simple, yet versatile, approach to generating skeletal muscle tissue of improved physiological relevance for applied and basic research. |
| Databáze: | OpenAIRE |
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