Development and Validation of Liquid Chromatography-Tandem Mass Spectrometry Methods for the Quantification of Cefquinome, Ceftiofur, and Desfuroylceftiofuracetamide in Porcine Feces with Emphasis on Analyte Stability
| Autor: | Mathias Devreese, Sofie Rutjens, Siska Croubels, Siegrid De Baere |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Male
0301 basic medicine Swine Antibiotics Pharmaceutical Science Organic chemistry Cefquinome 01 natural sciences Analytical Chemistry Matrix (chemical analysis) Feces QD241-441 desfuroylceftiofuracetamide Tandem Mass Spectrometry Liquid chromatography–mass spectrometry Acetamides Drug Discovery Chromatography High Pressure Liquid Observer Variation Chemistry Anti-Bacterial Agents (U)HPLC-MS Chemistry (miscellaneous) Calibration Molecular Medicine Female Ceftiofur medicine.drug cefquinome porcine feces Tazobactam Analyte medicine.drug_class 030106 microbiology Injections Intramuscular Article 03 medical and health sciences medicine Animals Veterinary Sciences Physical and Theoretical Chemistry Furans Chromatography 010401 analytical chemistry Organic Chemistry Reproducibility of Results Biology and Life Sciences method validation MS ceftiofur Cephalosporins 0104 chemical sciences (U)HPLC-MS/MS |
| Zdroj: | Molecules, Vol 26, Iss 4598, p 4598 (2021) MOLECULES Molecules Volume 26 Issue 15 |
| ISSN: | 1420-3049 |
| Popis: | Cefquinome and ceftiofur are β-lactam antibiotics used for the treatment of bacterial infections in swine. Although these antimicrobials are administered intramuscularly, the exposure of the gut microbiota to these cephalosporins is not well described. This exposure can contribute to the emergence and spread of antimicrobials in the environment and to the possible spread of antimicrobial resistance genes. To assess the impact of drug administration on the intestinal excretion of these antimicrobials it is essential to measure the amounts of native compound and metabolites in feces. Two (ultra)-high-performance liquid chromatography-tandem mass spectrometry ((U)HPLC–MS/MS) methods were developed and validated, one for the determination of cefquinome and ceftiofur and the other for the determination of ceftiofur residues, measured as desfuroylceftiofuracetamide, in porcine feces. The matrix-based calibration curve was linear from 5 ng g−1 to 1000 ng g−1 for cefquinome (correlation coefficient (r) = 0.9990 ± 0.0007 goodness of fit (gof) = 3.70 ± 1.43) and ceftiofur (r = 0.9979 ± 0.0009 gof = 5.51 ± 1.14) and quadratic from 30 ng g−1 to 2000 ng g−1 for desfuroylceftiofuracetamide (r = 0.9960 ± 0.0020 gof = 7.31 ± 1.76). The within-day and between-day precision and accuracy fell within the specified ranges. Since β-lactam antibiotics are known to be unstable in feces, additional experiments were conducted to adjust the sampling protocol in order to minimize the impact of the matrix constituents on the stability of the analytes. Immediately after sampling, 500 µL of an 8 µg mL−1 tazobactam solution in water was added to 0.5 g feces, to reduce the degradation in matrix. |
| Databáze: | OpenAIRE |
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