In vivo effects of neonatal administration of antiidiotype antibodies on experimental autoimmune myasthenia gravis
| Autor: | van Breda Vriesman Pj, De Baets Mh, Jan J.G.M. Verschuuren, Socrates J. Tzartos, Graus Ym |
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| Rok vydání: | 1991 |
| Předmět: |
Idiotype
Antiidiotype antibody medicine.drug_class Immunology Monoclonal antibody Torpedo Antigen Immunoglobulin Idiotypes Myasthenia Gravis medicine Immunology and Allergy Animals Receptors Cholinergic biology Antibody titer Fishes Antibodies Monoclonal medicine.disease Myasthenia gravis Antibodies Anti-Idiotypic Rats Disease Models Animal Animals Newborn Polyclonal antibodies Rats Inbred Lew biology.protein Female Antibody |
| Zdroj: | Autoimmunity. 10(3) |
| ISSN: | 0891-6934 |
| Popis: | The in vivo effects of neonatal administration of varying doses of anti-idiotype antibodies on serum anti-acetylcholine receptor (AChR) antibody titers, idiotype expression, and disease severity was studied in experimental autoimmune myasthenia gravis. Polyclonal affinity purified anti-idiotype antibodies and monoclonal anti-idiotype antibodies directed at anti-AChR monoclonal antibody 65 were administered in dosages varying from the nanogram to the microgram range. Mab 65 is directed against the main immunogenic region of mammalian AChR. In 1 out of 4 experiments administration of a nanogram dosage of anti-idiotype antibodies led to an enhanced anti-AChR antibody response after immunization with AChR. But no enhancing effect on idiotype expression could be demonstrated during this experiment. Adoptive transfer of spleen cells from rats pretreated with a nanogram dosage of anti-idiotype antibodies resulted in an significantly increased antibody response against rat AChR after immunization. From these experiments we conclude that in vivo administration of polyclonal or monoclonal anti-idiotypes does not reproduceably modify the serum antibody level against the acetylcholine receptor, nor influences the idiotype profile of the immune response. Secondly, the idiotype mediated manipulation of the immune response against large antigens, like the acetylcholine receptor, is clearly more complicated than that against small haptens. Adoptive transfer models, might be helpful in analysing the possibilities of anti-idiotype treatment in myasthenia gravis in more detail. |
| Databáze: | OpenAIRE |
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