HLH-14 is a C. elegans Achaete-Scute protein that promotes neurogenesis through asymmetric cell division
| Autor: | Gian Garriga, C. Andrew Frank, Paul D. Baum |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
endocrine system
Embryo Nonmammalian animal structures Cell division Molecular Sequence Data Nervous System Polymerase Chain Reaction Animals Genetically Modified Neuroblast hemic and lymphatic diseases Precursor cell Basic Helix-Loop-Helix Transcription Factors medicine Asymmetric cell division Animals Amino Acid Sequence Neurons Afferent Caenorhabditis elegans Caenorhabditis elegans Proteins Molecular Biology Conserved Sequence Body Patterning DNA Primers Motor Neurons Neurons Genetics Base Sequence Sequence Homology Amino Acid biology Helix-Loop-Helix Motifs fungi Neurogenesis biology.organism_classification Sensory neuron medicine.anatomical_structure nervous system Multigene Family Scute Sequence Alignment Cell Division Gene Deletion Transcription Factors Developmental Biology |
| Zdroj: | Development. 130:6507-6518 |
| ISSN: | 1477-9129 0950-1991 |
| DOI: | 10.1242/dev.00894 |
| Popis: | Achaete-Scute basic helix-loop-helix (bHLH) proteins promote neurogenesis during metazoan development. In this study, we characterize a C. elegans Achaete-Scute homolog, HLH-14. We find that a number of neuroblasts express HLH-14 in the C. elegans embryo, including the PVQ/HSN/PHB neuroblast, a cell that generates the PVQ interneuron, the HSN motoneuron and the PHB sensory neuron. hlh-14 mutants lack all three of these neurons. The fact that HLH-14 promotes all three classes of neuron indicates that C. elegans proneural bHLH factors may act less specifically than their fly and mammalian homologs. Furthermore, neural loss in hlh-14 mutants results from a defect in an asymmetric cell division: the PVQ/HSN/PHB neuroblast inappropriately assumes characteristics of its sister cell, the hyp7/T blast cell. We argue that bHLH proteins, which control various aspects of metazoan development, can control cell fate choices in C. elegans by regulating asymmetric cell divisions. Finally, a reduction in the function of hlh-2, which encodes the C. elegans E/Daughterless bHLH homolog, results in similar neuron loss as hlh-14 mutants and enhances the effects of partially reducing hlh-14 function. We propose that HLH-14 and HLH-2 act together to specify neuroblast lineages and promote neuronal fate. |
| Databáze: | OpenAIRE |
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