Cigarette smoke and glutathione: Focus on in vitro cell models
| Autor: | Maria Chiara Lionetti, Isabella Dalle-Donne, Daniela Giustarini, Ranieri Rossi, Maria Lisa Garavaglia, Emanuela Astori, Graziano Colombo, Caterina A. M. La Porta, Annalisa Santucci, Aldo Milzani |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Cell Toxicology medicine.disease_cause Models Biological 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Smoke Oxidative damage Tobacco Cigarette smoke Glutathione in vitro cell models Oxidative stress Smokers medicine Animals Humans Respiratory system chemistry.chemical_classification Chemistry Cancer General Medicine medicine.disease In vitro 030104 developmental biology medicine.anatomical_structure Enzyme Biochemistry 030220 oncology & carcinogenesis Intracellular |
| Zdroj: | Toxicology in vitro : an international journal published in association with BIBRA. 65 |
| ISSN: | 1879-3177 |
| Popis: | Cigarette smoke (CS) is one of the most important preventable risk factors for the development of respiratory diseases, cardiovascular diseases, stroke, and various types of cancer. Due to its high intracellular concentration and central role in maintaining the cellular redox state, glutathione (GSH) is one of the key players in several enzymatic and non-enzymatic reactions necessary for protecting cells against CS-induced oxidative stress. A plethora of in vitro cell models have been used over the years to assess the effects of CS on intracellular GSH and its disulphide forms, i.e. glutathione disulphide (GSSG) and S-glutathionylated proteins. In this review, we described the effects of cell exposure to CS on cellular GSH and formation of its oxidized forms and adducts (GSH-conjugates). We also discussed the limitations and relevance of in vitro cell models of exposure to CS and critically assessed the congruence between smokers and in vitro cell models. What emerges clearly is that results obtained in vitro should be interpreted with extreme caution, bearing in mind the limitations of the specific cell model used. Despite this, in vitro cell models remain important tools in the assessment of CS-induced oxidative damage. |
| Databáze: | OpenAIRE |
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