Screening, mutagenesis and protoplast fusion of Aspergillus niger for the enhancement of extracellular glucose oxidase production
| Autor: | A. A. Khattab, W. A. Bazaraa |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Genotype
biology Strain (chemistry) Protoplasts Aspergillus niger Mutant Mutagenesis (molecular biology technique) Bioengineering Methane sulfonate Protoplast biology.organism_classification Applied Microbiology and Biotechnology humanities Fungal Proteins Glucose Oxidase Biochemistry Mutagenesis Extracellular biology.protein Glucose oxidase Biotechnology |
| Zdroj: | Journal of Industrial Microbiology & Biotechnology. 32:289-294 |
| ISSN: | 1476-5535 1367-5435 |
| DOI: | 10.1007/s10295-005-0249-7 |
| Popis: | Various strains of Aspergillus niger were screened for extracellular glucose oxidase (GOD) activity. The most effective producer, strain FS-3 (15.9 U mL(-1)), was mutagenized using UV-irradiation or ethyl methane sulfonate. Of the 400 mutants obtained, 32 were found to be resistant to 2-deoxy D: -glucose, and 17 of these exhibited higher GOD activities (from 114.5 to 332.1%) than the original FS-3 strain. Following determination of antifungal resistance of the highest producing mutants, four mutants were selected and used in protoplast fusions in three different intraspecific crosses. All fusants showed higher activities (from 285.5 to 394.2%) than the original strain. Moreover, of the 30 fusants isolated, 19 showed higher GOD activity than their corresponding higher-producing parent strain. |
| Databáze: | OpenAIRE |
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