Monocyte monolayer assay in pre-transfusion testing: A magic key in transfusing patients with recurrent bad cross-match due to alloimmunization
| Autor: | Hebat Allah N. El-sayed, Dina Ragab, Shereen N. Raafat, Maha R.A. Abdollah |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Blood transfusion medicine.medical_treatment Immunology Cell Culture Techniques Giemsa stain Monocytes 03 medical and health sciences 0302 clinical medicine Antigen Isoantibodies Immunology and Allergy Medicine Humans biology business.industry Monocyte Autoantibody Transfusion Reaction Staining Agglutination (biology) 030104 developmental biology medicine.anatomical_structure Blood Grouping and Crossmatching biology.protein Blood Group Antigens Antibody business 030215 immunology |
| Zdroj: | Journal of immunological methods. 492 |
| ISSN: | 1872-7905 |
| Popis: | The monocyte monolayer assay (MMA) is an in-vitro assay that can predict the outcome of blood transfusion of antigen positive units when serologically compatible blood is not available.Fifty-four patients testing positive by the antibody screening test using gel agglutination were further examined by the alloantibody identification panel to determine alloantibody specificity. After determining and categorizing the antibodies, patients' samples were examined using the MMA to determine the clinical significance of the detected alloantibodies. We also tested 2 seeding methods (24-well cell culture plates versus 8-well chamber-slides) and 3 visualization/staining techniques (unstained phase contrast, Leishman and Giemsa staining).35 out of the 54 cases (64.8%) had a monocyte index of5% which is predictive of occurrence of hemolytic reaction after transfusion; 23 cases with antibodies known to be clinically significant [anti-C, anti-E, anti-c, anti-K, anti-Fy(a), anti Fy(b), anti-JK(b)], 2 with Anti-M specificity, 7 cases with autoantibodies and 3 cases with multiple antibodies. On the other hand, 19 out of the 54 (35.2%) cases included in the study showed a monocyte index of5% which is predictive of absence of hemolytic reaction after transfusion. The 8-well chamber-slides were better than the 24-well culture plates, as the latter showed a lot of un-phagocytosed RBCs in the background. Also, Leishman staining was better than Giemsa staining with better and clearer differentiation between the RBCs, monocytes and phagocytic vacuoles.MMA can be used as a surrogate cross-match test for the selection of blood units in cases where antigen-negative blood units are not available. |
| Databáze: | OpenAIRE |
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