Reduction of Aluminum Toxicity by 2-Isopropylmalic Acid in the Budding Yeast Saccharomyces cerevisiae
| Autor: | Etsuro Yoshimura, Naoko K. Nishizawa, Mitsuru Tashiro, Taisuke Suzuki, Shun Tamura, Hiromi Nakanishi |
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| Rok vydání: | 2007 |
| Předmět: |
Saccharomyces cerevisiae Proteins
Endocrinology Diabetes and Metabolism Clinical Biochemistry Saccharomyces cerevisiae Mutant Malates Biochemistry Inorganic Chemistry chemistry.chemical_compound Chlorides Aluminum Chloride Chelation Secretion Aluminum Compounds biology Biochemistry (medical) Wild type General Medicine biology.organism_classification Yeast chemistry Toxicity 2-Isopropylmalate Synthase Malic acid |
| Zdroj: | Biological Trace Element Research. 120:257-263 |
| ISSN: | 1559-0720 0163-4984 |
| DOI: | 10.1007/s12011-007-8011-9 |
| Popis: | The budding yeast Saccharomyces cerevisiae secretes 2-isopropylmalic acid (2-iPMA), an intermediate in leucine biosynthesis. Because 2-iPMA binds Al(III) in the culture medium, it is thought to reduce toxicity by Al(III). The effects of 2-iPMA and malic acid (MA) on Al toxicity were investigated in a medium with a low pH and low concentrations of phosphates and magnesium. The reduction in the growth of S. cerevisiae observed in the presence of 100 muM Al(III) ions was relieved more by the addition of 1.0 mM 2-iPMA than by 1.0 mM MA, indicating that 2-iPMA possesses superior Al(III)-ion detoxification ability. Investigations using the wild type and the deltaleu4 and deltaleu9 mutant strains indicated that secretion of a sufficient level of 2-iPMA was required to enhance the Al tolerance. It is thought that 2-iPMA secreted from the yeast cells chelates Al ions and prevents them from entering the cells, resulting in Al tolerance. |
| Databáze: | OpenAIRE |
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