The preventive effect of diphenhydramine on bacterial growth in propofol
| Autor: | H. Yilmaz, V. Turhan, S. Apiliogullari, M. Balasar, Ates Duman, B. Apiliogullari, A. Güzelant, Inci Kara |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Staphylococcus aureus
Lidocaine Bacterial growth Pharmacology medicine.disease_cause Agar plate Candida albicans Escherichia coli medicine Anesthetics Local Propofol Analysis of Variance Dose-Response Relationship Drug biology business.industry Pseudomonas aeruginosa Diphenhydramine biology.organism_classification Antimicrobial Anesthesiology and Pain Medicine Anesthesia Drug Contamination business Anesthetics Intravenous medicine.drug |
| Zdroj: | European Journal of Anaesthesiology. 25:737-740 |
| ISSN: | 0265-0215 |
| DOI: | 10.1017/s0265021508004377 |
| Popis: | Background and objectiveDiphenhydramine has local anaesthetic and antimicrobial activity and may be used to prevent intravenous propofol injection pain. We studied the effect of adding diphenhydramine to propofol emulsions for preventing bacterial growth.MethodsStaphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli and Candida albicans cultures were inoculated into the following solutions: 1% propofol, 0.05% diphenhydramine + 1% propofol, 0.1% diphenhydramine + 1% propofol, 0.2% diphenhydramine + 1% propofol, 0.3% diphenhydramine + 1% propofol, 1% diphenhydramine and 0.1% lidocaine + 1% propofol. A 100-μL of inoculum suspension adjusted for each of the micro-organisms was added separately to each tube and left at 20°C. A 10-μL aliquot of each mixture was inoculated onto blood agar medium at 5 and 24 h. These plates were incubated at 35°C for 24 h. Each plated medium was read, and the number of colony-forming units were counted and recorded (n = 2). Analysis of variance (ANOVA) with a post hoc Tukey HSD test and paired t-tests were used for data analysis. P < 0.05 was considered as significant.ResultsDiphenhydramine inhibited bacterial growth in propofol solutions in a dose-dependent manner. It was more effective than 0.1% lidocaine at similar concentrations in preventing bacterial growth for all organisms.ConclusionDiphenhydramine had a significant inhibitory effect on bacterial growth in propofol. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|