Effect of Trichostatin A on miRNA expression in cultures of primary rat hepatocytes
| Autor: | Daneida Lizarraga, Joost H.M. van Delft, Tamara Vanhaecke, Jennifer Bolleyn, Joanna Fraczek, Vera Rogiers, Stan Gaj, Mathieu Vinken |
|---|---|
| Přispěvatelé: | Experimental in vitro toxicology and dermato-cosmetology, Toxicology, Dermato-cosmetology and Pharmacognosy, RS: NUTRIM - R4 - Gene-environment interaction, Toxicogenomics, RS: GROW - School for Oncology and Reproduction |
| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
Male
Untranslated region CYCLIN G1 medicine.drug_class LIVER-REGENERATION Down-Regulation Biology Hydroxamic Acids Toxicology Rats Sprague-Dawley PCR DATA microRNA medicine Animals Epigenetics Cells Cultured Cell Proliferation Histone deacetylase inhibitor IDENTIFICATION Microarray analysis techniques MICRORNA General Medicine IN-VITRO Microarray Analysis Molecular biology CANCER In vitro Liver regeneration Rats Up-Regulation Histone Deacetylase Inhibitors MicroRNAs Trichostatin A DIFFERENTIATION CELLS Hepatocytes GROWTH Chemical and Drug Induced Liver Injury medicine.drug |
| Zdroj: | Vrije Universiteit Brussel Toxicology in Vitro, 25(6), 1173-1182. Elsevier Science |
| ISSN: | 0887-2333 |
| DOI: | 10.1016/j.tiv.2011.04.013 |
| Popis: | In the present study, the effect of Trichostatin A (TSA), a histone deacetylase inhibitor, was investigated on the microRNA (miR, miRNA) expression profile in cultured primary rat hepatocytes by means of microarray analysis. Simultaneously, albumin secretory capacity and morphological features of the hepatocytes were evaluated throughout the culture time. In total, 25 out of 348 miRNAs were found to be differentially expressed between freshly isolated hepatocytes and 7-day cultured cells. Nineteen of these miRNAs were connected with 'general metabolism'. MiR-21 and miR-126 were shown to be the most up and down regulated miRs upon cultivation and could be linked to the proliferative response triggered in the hepatocytes upon their isolation from the liver. MiR-379 and miR-143, on the other hand, were found to be the most up and down regulated miRs upon TSA treatment. Together with the higher expression of miR-122 observed in TSA-treated versus non-treated cultures, we hypothesise that the changes observed formiR-122, miR-143 and miR-379 could be related to the inhibitory effects of TSA on hepatocecellular proliferation. |
| Databáze: | OpenAIRE |
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