Aged insulin granules display reduced microtubule-dependent mobility and are disposed within actin-positive multigranular bodies
| Autor: | Martina Lachnit, Andreas Müller, Hassan Mziaut, Michael Meyer-Hermann, Jaber Dehghany, Yannis Kalaidzidis, Peter Hoboth, Anna Ivanova, Michele Solimena, Anke Sönmez |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
medicine.medical_specialty
medicine.medical_treatment Biology Microtubules Corrections Glucose stimulation stomatognathic system Microtubule Internal medicine Cell Line Tumor medicine Glucose homeostasis Animals Insulin Secretion Actin Cellular Senescence Multidisciplinary Microscopy Confocal Secretory Vesicles Secretory Vesicle Actins Cell biology Bayesian Probability Theory Diabetes Islets Processivity Rats Endocrinology PNAS Plus Cell aging |
| Zdroj: | Proc. Natl. Acad. Sci. U.S.A. 112, E667-E676 (2015) |
| ISSN: | 1091-6490 |
| Popis: | Insulin secretion is key for glucose homeostasis. Insulin secretory granules (SGs) exist in different functional pools, with young SGs being more mobile and preferentially secreted. However, the principles governing the mobility of age-distinct SGs remain undefined. Using the time-reporter insulin-SNAP to track age-distinct SGs we now show that their dynamics can be classified into three components: highly dynamic, restricted, and nearly immobile. Young SGs display all three components, whereas old SGs are either restricted or nearly immobile. Both glucose stimulation and F-actin depolymerization recruit a fraction of nearly immobile young, but not old, SGs for highly dynamic, microtubule-dependent transport. Moreover, F-actin marks multigranular bodies/lysosomes containing aged SGs. These data demonstrate that SGs lose their responsiveness to glucose stimulation and competence for microtubule-mediated transport over time while changing their relationship with F-actin. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|