Effects of fish oil on methotrexate-induced reproductive damage in rats

Autor:	Volkan Ipek, Kursat Kaya, Cigdem Cebi, Ali Gurel, Leyla Elif Ozgu Ayozger
Rok vydání:	2022
Předmět:	reproductive toxicity Male antioxidant sperm quality Urology thiobarbituric acid reactive substance animal experiment animal cell sperm count fish oil sperm viability sperm Thiobarbituric Acid Reactive Substances Article Antioxidants animal tissue histology Endocrinology Fish Oils biochemical analysis Semen caspase 3 Animals controlled study rat animal glutathione peroxidase semen parameters nonhuman Superoxide Dismutase animal model spermatozoon motility catalase apoptosis germ cell General Medicine general anesthesia Glutathione enzyme activity Rats Oxidative Stress Methotrexate immunohistochemistry histopathology Sperm Motility metabolism
Zdroj:	AndrologiaREFERENCES. 54(11)
ISSN:	1439-0272
Popis:	Methotrexate (MTX) is a folic acid antagonist that is commonly used in paediatric and adult oncology to treat a variety of malignancies. Internal organs, including the testis, are severely cytotoxic and genotoxic to MTX. Omega-3, as an antioxidant, has been shown to protect rat testis tissue from injury. The effect of fish oil (FO) on MTX-induced reproductive damage in rats was investigated in this work. The 28 animals were divided into four groups for this purpose (control, FO, MTX, and MTX-FO). On the third day, the MTX group received a single intraperitoneal injection of 20 mg/kg MTX. Furthermore, in the FO and MTX-FO groups, FO was delivered through gavage once daily for 14 days. All animals euthanized under general anaesthesia on the 15th day. TBARS, catalase, glutathione peroxidase, glutathione (GSH), superoxide dismutase levels were measured biochemically. The Cosentino grading system was utilized for histology. Germ cell thickness and caspase-3 activity were also evaluated. In addition, sperm motility rate, epididymal sperm count, aberrant sperm rate, and sperm vitality were measured to assess sperm quality. Some TBARS levels have increased, but GSH levels decreased significantly in the MTX group. FO reduced TBARS levels while considerably increasing GSH levels. All sperm quality measures were significantly lowered in the MTX group, while FO had a recovery effect. There were no notable variations in histopathology across groups except for germ cell thickness, which reduced considerably in the MTX group and recovered with FO treatment. As a result, FO has been shown to reduce testicular toxicity following MTX treatment in rats. © 2022 Wiley-VCH GmbH.
Databáze:	OpenAIRE
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