Genetic diversity patterns and functional traits of bradyrhizobium strains associated with Pterocarpus officinalis Jacq. in Caribbean Islands and Amazonian Forest (French Guiana)
Autor: | Antoine Galiana, Gilles Béna, Christine Le Roux, Jean-Marc Bouvet, Bernard Dreyfus, Amadou Moustapha Bâ, Félix Muller |
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Jazyk: | angličtina |
Rok vydání: | 2014 |
Předmět: |
Root nodule
F62 - Physiologie végétale - Croissance et développement Évolution Forests Mycorhizé à vésicule et arbuscule Interactions biologiques RNA Ribosomal 16S K01 - Foresterie - Considérations générales Bradyrhizobium Forêt tropicale humide Phylogeny Ecology biology food and beverages French Guiana RNA Ribosomal 23S Officinalis Root Nodules Plant DNA Bacterial Reconstitution forestière Pterocarpus West Indies Symbiose Soil Science Variation génétique Symbiosis DNA Ribosomal Spacer Botany Ecology Evolution Behavior and Systematics Genetic diversity Host (biology) Genetic Variation P34 - Biologie du sol Sequence Analysis DNA biology.organism_classification Sol marécageux Aptitude à coloniser Bradyrhizobium japonicum |
Zdroj: | Microbial Ecology |
Popis: | Pterocarpus officinalis Jacq. is a legume tree native to the Caribbean islands and South America growing as a dominant species in swamp forests. To analyze (i) the genetic diversity and (ii) the symbiotic properties of its associated nitrogen-fixing soil bacteria, root nodules were collected from P. officinalis distributed in 16 forest sites of the Caribbean islands and French Guiana. The sequencing of the 16S-23S ribosomal RNA intergenic spacer region (ITS) showed that all bacteria belonged to the Bradyrhizobium genus. Bacteria isolated from insular zones showed very close sequence homologies with Bradyrhizobium genospecies V belonging to the Bradyrhizobium japonicum super-clade. By contrast, bacteria isolated from continental region displayed a larger genetic diversity and belonged to B. elkanii super-clade. Two strains from Puerto Rico and one from French Guiana were not related to any known sequence and could be defined as a new genospecies. Inoculation experiments did not show any host specificity of the Bradyrhizobium strains tested in terms of infectivity. However, homologous Bradyrhizobium sp. strain-P. officinalis provenance associations were more efficient in terms of nodule production, N acquisition, and growth than heterologous ones. The dominant status of P. officinalis in the islands may explain the lower bacterial diversity compared to that found in the continent where P. officinalis is associated with other leguminous tree species. The specificity in efficiency found between Bradyrhizobium strains and host tree provenances could be due to a coevolution process between both partners and needs to be taken in consideration in the framework of rehabilitation plantation programs. |
Databáze: | OpenAIRE |
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