MiRNA expression profiling in human gliomas: upregulated miR-363 increases cell survival and proliferation
| Autor: | Francesca Polito, Filippo Flavio Angileri, Rosamaria Di Giorgio, Gaetana Pezzino, Valeria Barresi, Annamaria Cama, S. Romeo, M'hammed Aguennouz, Guido Ferlazzo, Domenico La Torre, Alfredo Conti, Antonino Germanò, Salvatore Cardali, Chiara Tomasello |
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| Přispěvatelé: | CONTI, Alfredo, ROMEO, SARA GIOVANNA, CAMA, annamaria, LA TORRE, Domenico, BARRESI, Valeria, PEZZINO, GAETANA, TOMASELLO, CHIARA, CARDALI, Salvatore Massimiliano, Angileri, Filippo F., POLITO, FRANCESCA, FERLAZZO, Guido, DI GIORGIO, Rosa Maria, GERMANO', Antonino Francesco, AGUENNOUZ, M'hammed |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Male Cancer Research Messenger Apoptosis Immunoenzyme Techniques 0302 clinical medicine GAP-43 Protein Tumor Cells Cultured Regulation of gene expression Tumor Cultured Blotting Brain Neoplasms Reverse Transcriptase Polymerase Chain Reaction Medicine (all) Astrocytoma rap1 GTP-Binding Proteins General Medicine Transfection Glioma Middle Aged Prognosis Tumor Cells Gene Expression Regulation Neoplastic Survival Rate 030220 oncology & carcinogenesis Gap-43 Western Glioblastoma multiforme miRNA microarray miRNA-363 Adult Aged Biomarkers Follow-Up Studies Histone Deacetylases Humans MicroRNAs Neoplasm Grading Neoplasm Invasiveness RNA Real-Time Polymerase Chain Reaction Repressor Proteins Cell Proliferation Gene Expression Regulation Neoplastic Blotting Western Biology 03 medical and health sciences Downregulation and upregulation microRNA Biomarkers Tumor medicine RNA Messenger neoplasms Cell growth Astrocytoma Gap-43 Glioblastoma multiforme miRNA microarray miRNA-363 Adult Aged Biomarkers Tumor Blotting Western Brain Neoplasms Follow-Up Studies GAP-43 Protein Glioma Histone Deacetylases Humans Immunoenzyme Techniques Male MicroRNAs Middle Aged Neoplasm Grading Neoplasm Invasiveness Prognosis RNA Messenger Real-Time Polymerase Chain Reaction Repressor Proteins Reverse Transcriptase Polymerase Chain Reaction Survival Rate Tumor Cells Cultured rap1 GTP-Binding Proteins Apoptosis Cell Proliferation medicine.disease Molecular biology nervous system diseases 030104 developmental biology Cell culture |
| Popis: | The role of microRNAs (miRNAs) in glioma biology is increasingly recognized. To investigate the regulatory mechanisms governing the malignant signature of gliomas with different grades of malignancy, we analyzed miRNA expression profiles in human grade I–IV tumor samples and primary glioma cell cultures. Multiplex real-time PCR was used to profile miRNA expression in a set of World Health Organization (WHO) grade I (pilocytic astrocytoma), II (diffuse fibrillary astrocytoma), and IV (glioblastoma multiforme) astrocytic tumors and primary glioma cell cultures. Primary glioma cell cultures were used to evaluate the effect of transfection of specific miRNAs and miRNA inhibitors. miRNA microarray showed that a set of miRNAs was consistently upregulated in all glioma samples. miR-363 was upregulated in all tumor specimens and cell lines, and its expression correlated with tumor grading. The transfection of glioma cells with the specific inhibitor of miR-363 increased the expression level of tumor suppressor growth-associated protein 43 (GAP-43). Transfection of miR-363 induced cell survival, while inhibition of miR-363 significantly reduced glioma cell viability. Furthermore, miRNA-363 inhibition induced the downregulation of AKT, cyclin-D1, matrix metalloproteinase (MMP)-2, MMP-9, and Bcl-2 and upregulation of caspase 3. Together, these data suggest that the upregulation of miR-363 may play a role in malignant glioma signature. |
| Databáze: | OpenAIRE |
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