Piperine-loaded chitosan-STPP nanoparticles reduce neuronal loss and astrocytes activation in chemical kindling model of epilepsy
| Autor: | Mona Hashemian, Maryam Ghasemi-Kasman, Ali Akbar Moghadamnia, Atefeh Akbari, Sohrab Kazemi, Diana Anissian, Mohsen Khalili-Fomeshi |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Polyunsaturated Alkamides medicine.medical_treatment Pharmacology Biochemistry Neuroprotection 03 medical and health sciences chemistry.chemical_compound Epilepsy Mice 0302 clinical medicine Alkaloids Organophosphorus Compounds Piperidines Structural Biology medicine Kindling Neurologic Animals Humans Benzodioxoles Molecular Biology Neurons Chitosan biology Alkaloid technology industry and agriculture General Medicine medicine.disease Disease Models Animal 030104 developmental biology Anticonvulsant chemistry Piperine Astrocytes biology.protein Nanoparticles Pentylenetetrazole NeuN Kindling model Hydrophobic and Hydrophilic Interactions 030217 neurology & neurosurgery Immunostaining Stearic Acids |
| Zdroj: | International journal of biological macromolecules. 107 |
| ISSN: | 1879-0003 |
| Popis: | Recent evidence suggests that encapsulation of hydrophobic drugs in biodegradable polymers opens a new horizon in nanomedicine filed. Piperine, a main alkaloid form of black pepper possesses potent anticonvulsant activity. However, the low water solubility of piperine has limited its clinical application. In this study, piperine was loaded on chitosan-sodium tripolyphosphate nanoparticles (CS-STPP NPs) and the effect of piperine NPs on seizures behavior and astrocytes activation was assessed in pentylentetrazol (PTZ)-induced kindling model. Animals have received the daily injection of free piperine or piperine NPs at doses of 5 or 10mg/kg, 10days before PTZ injections and their intraperitoneally (i.p) administration continued until the last PTZ injection. The neuroprotective effects of piperine NPs were evaluated using nissl staining and immunostaining against NeuN. Astrocytes activation was examined by GFAP immunostaining. Behavioral data showed that piperine NPs have inhibited the development of seizure parameters compared to the free piperine groups. In addition, the levels of cell loss and astrocytes activation were reduced in piperine NPs groups. In conclusion, these data suggest that piperine NPs enhance the neuroprotection and ameliorate the astrocytes activation in chemical kindling model of epilepsy. This may provide an effective therapeutic strategy for the treatment of epilepsy disorder. |
| Databáze: | OpenAIRE |
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