Sex-specific effects of low protein diet on in utero programming of renal G-protein coupled receptors
| Autor: | Stefan Gysler, Timothy R.H. Regnault, L. Zhao, C.-L. Cooke, Edith Arany |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Male
medicine.medical_specialty Angiotensin receptor Low protein Offspring medicine.medical_treatment Medicine (miscellaneous) Kidney Receptors G-Protein-Coupled Sex Factors Low-protein diet Pregnancy Internal medicine Diet Protein-Restricted medicine Animals Rats Wistar Prenatal Nutritional Physiological Phenomena Fetal Growth Retardation biology business.industry Kidney metabolism Angiotensin-converting enzyme Endocrinology medicine.anatomical_structure In utero biology.protein Female business |
| Zdroj: | Journal of Developmental Origins of Health and Disease. 5:36-44 |
| ISSN: | 2040-1752 2040-1744 |
| DOI: | 10.1017/s2040174413000524 |
| Popis: | Intrauterine growth restriction (IUGR) is an important risk factor for development of hypertension, diabetes and the metabolic syndrome. Maternal low protein (LP) intake during rat pregnancy leads to IUGR in male and female offspring, although females may be resistant to the development of effect. Current evidence suggests that changes in the renin-angiotensin system (RAS) in utero contribute to this programmed hypertension, via sex-specific mechanisms. The previously orphaned G-protein coupled receptor (GPR91) was identified as a central player in the development of hypertension in adult mice, through a RAS-dependent pathway. However, whether the GPR91 pathway contributes to fetal programming is unknown. Furthermore, the nature of involvement of downstream modulators of the RAS including Gqα/11α and GαS has not been investigated in IUGR-LP rats. Therefore, we postulated that renal GPR91, in conjunction with RAS, is differentially impacted in a sex-specific manner from LP-induced IUGR rats. Pregnant Wistar rats were fed control (C, 20% protein) or LP (8% protein) diet until embryonic day 19 (E19) or postnatal d21. At E19, GPR91 protein and mRNA were increased in both male and female LP kidneys (PP=0.06 and P1R and Gqα/11α were increased in LP males, while in LP females, AT2R protein was elevated and renin expression was decreased (Pin utero alterations, when combined with postnatal increases in AT1R-Gqα/11α specifically in male offspring, may predispose to the development of hypertension. |
| Databáze: | OpenAIRE |
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