Genome-Wide Identification, Characterization, and Expression Analysis of Tubby-like Protein (TLP) Gene Family Members in Woodland Strawberry (
| Autor: | Shuangtao Li, Guixia Wang, Linlin Chang, Rui Sun, Ruishuang Wu, Chuanfei Zhong, Yongshun Gao, Hongli Zhang, Lingzhi Wei, Yongqing Wei, Yuntao Zhang, Jing Dong, Jian Sun |
|---|---|
| Rok vydání: | 2022 |
| Předmět: |
Organic Chemistry
Arabidopsis General Medicine Sodium Chloride Fragaria Catalysis Hormones Computer Science Applications Inorganic Chemistry Gene Expression Regulation Plant tubby-like proteins Fragaria vesca abiotic stress evolution pattern expression analysis Physical and Theoretical Chemistry Molecular Biology Spectroscopy Abscisic Acid Glucuronidase Plant Proteins |
| Zdroj: | International Journal of Molecular Sciences; Volume 23; Issue 19; Pages: 11961 |
| ISSN: | 1422-0067 |
| Popis: | Tubby-like proteins (TLPs) play important roles in plant growth and development and in responses to abiotic stress. However, TLPs in strawberry remain poorly studied. In this study, eight TLPs were identified in woodland strawberry (Fragaria vesca subspecies vesca ‘Ruegen’). Protein structure analysis revealed that the structure of FvTLPs is highly conserved, but evolutionary and gene structure analyses revealed that the evolutionary pattern of FvTLP family members differs from that of their orthologous genes in Arabidopsis, poplar, and apple. Subcellular localization assays revealed that FvTLPs were localized to the nucleus and plasma membrane. FvTLPs showed no transcriptional activity. Yeast two-hybrid assays revealed that FvTLPs interact with specific FvSKP1s. The expression patterns of FvTLPs in different tissues and under various abiotic stresses (salt, drought, cold, and heat) and hormone treatments (ABA (abscisic acid) and MeJA (methyl jasmonate)) were determined. The expression patterns of FvTLPs indicated that they play a role in regulating growth and development and responses to abiotic stress in F. vesca. The GUS (beta-glucuronidase) activity of FvTLP1pro::GUS plants in GUS activity assays increased under salt and drought stress and abscisic acid treatment. The results of this study provide new insights into the molecular mechanisms underlying the functions of TLPs. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|