Altered expression of CD11b/CD18 and CD62L on human monocytes after cell preparation procedures
| Autor: | Joachim Lundahl, M. Hallgren, C.M. Sköld, Gunilla Halldén, J. Hed |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Adult
Lysis Adolescent Immunology Cell Separation Platelet Membrane Glycoproteins Peripheral blood mononuclear cell Monocytes Flow cytometry chemistry.chemical_compound Antigens CD Centrifugation Density Gradient medicine Humans Immunology and Allergy Aged Differential centrifugation biology medicine.diagnostic_test CD11 Antigens Monocyte Middle Aged Flow Cytometry Molecular biology P-Selectin medicine.anatomical_structure Integrin alpha M chemistry Biochemistry CD18 Antigens biology.protein Ammonium chloride Ex vivo |
| Zdroj: | Journal of Immunological Methods. 180:93-100 |
| ISSN: | 0022-1759 |
| Popis: | We have investigated the effect of different cell preparation procedures on the surface expression of CD11b/CD18 and CD62L on human monocytes. Both EDTA and heparin anticoagulated blood were used as sources for leukocytes. The monocytes were analysed by flow cytometry in a mixed leukocyte suspensions obtained after ammonium chloride mediated lysis and in mononuclear cell suspension prepared by density gradient centrifugation (Ficoll-Paque) performed both at 4 degrees C and at 20 degrees C. Monocytes from heparinized blood had a higher expression of CD11b/CD18 and a more pronounced inter-individual variation than monocytes from EDTA blood. Monocytes isolated by Ficoll-Paque had a higher degree of ex vivo activation by means of altered expression of CD11b/CD18 and CD62L compared to monocytes prepared by ammonium chloride mediated lysis. This was more pronounced when the isolation procedure was performed at 20 degrees C. Our findings indicate that monocytes prepared by ammonium chloride mediated lysis of EDTA blood and with the cell handling temperature kept at 4 degrees C are exposed to the smallest ex vivo modulation by means of receptor alteration. An awareness of ex vivo modulation by different cell preparation procedures is of importance especially when comparing the expression of functional receptors on leukocytes of disparate origin. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect