Dicer knockdown induces fibronectin-1 expression in HEK293T cells via induction of Egr1
| Autor: | Guan-Bin Song, Yi-Song Shi, Kai-Fu Tang, Lin Yuan, Yong-Hua Li |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Ribonuclease III
Small interfering RNA Blotting Western genetic processes Biophysics Gene Expression Apoptosis Enzyme-Linked Immunosorbent Assay Kidney Biochemistry Cell Line Downregulation and upregulation Cell Movement microRNA Humans RNA Small Interfering Molecular Biology DNA Primers Early Growth Response Protein 1 Gene knockdown biology Reverse Transcriptase Polymerase Chain Reaction fungi HEK 293 cells Transfection Molecular biology Chromatin Fibronectins Up-Regulation enzymes and coenzymes (carbohydrates) Gene Knockdown Techniques biology.protein Dicer |
| Zdroj: | Biochimica et Biophysica Acta (BBA) - General Subjects. 1800:380-384 |
| ISSN: | 0304-4165 |
| Popis: | Background Dicer is a multidomain ribonuclease III enzyme involved in the biogenesis of microRNAs (miRNAs) and small interfering RNAs (siRNAs); depletion of Dicer was found to impair the migration of endothelial cells. Methods siRNA transfection, cell migration assay, real-time RT–PCR, chromatin immunoprecipitation, Western blotting, ELISA, caspase-3 activity assay, and annexin-V–FITC assay were utilized. Results Knockdown of Dicer impairs the migratory capacity of HEK293T cells and induces fibronectin-1. The upregulation of fibronectin-1 is dependent on Egr1. Fibronectin-1/Dicer double-knockdown cells showed a marked increase in apoptosis compared with fibronectin-1 single knockdown cells. Conclusions Decreased Dicer expression induces fibronectin-1 expression via an Egr1-dependent manner. General significance Our data suggest that upregulation of fibronectin-1 protects Dicer knockdown HEK293T cells against apoptosis. |
| Databáze: | OpenAIRE |
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