KLRG1 Impairs CD4+ T Cell Responses via p16ink4a and p27kip1 Pathways: Role in Hepatitis B Vaccine Failure in Individuals with Hepatitis C Virus Infection
| Autor: | Jeddidiah W. D. Griffin, Jia M. Wang, Zhi Q. Yao, Guang Y. Li, Shu M. Lin, Ruo S. Ying, Jun P. Ren, Yong Q. Cheng, Lei Shi, Jonathan P. Moorman, Xiao Y. Wu |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
CD4-Positive T-Lymphocytes
Interleukin 2 Aging Hepatitis B virus Hepatitis B vaccine Hepatitis C virus T cell Immunology Hepacivirus Biology medicine.disease_cause Article Cyclin E medicine Humans Immunology and Allergy Hepatitis B Vaccines Lectins C-Type Phosphorylation Receptors Immunologic Cells Cultured Cyclin-Dependent Kinase Inhibitor p16 Cell Proliferation Coinfection Cyclin-Dependent Kinase 2 virus diseases Hepatitis C Hepatitis B medicine.disease Virology digestive system diseases medicine.anatomical_structure Trans-Activators Interleukin-2 Proto-Oncogene Proteins c-akt Cyclin-Dependent Kinase Inhibitor p27 Signal Transduction medicine.drug |
| Zdroj: | The Journal of Immunology. 192:649-657 |
| ISSN: | 1550-6606 0022-1767 |
| DOI: | 10.4049/jimmunol.1302069 |
| Popis: | Coinfection of hepatitis B virus (HBV) with hepatitis C virus (HCV) is quite common, leading to an increase in morbidity and mortality. As such, HBV vaccination is recommended in HCV-infected individuals. However, HBV vaccine responses in HCV-infected individuals are often blunted compared with uninfected populations. The mechanism for this failure of vaccine response in HCV-infected subjects remains unclear. In this study, we investigated the expression and function of an inhibitory receptor, killer cell lectin-like receptor subfamily G member 1 (KLRG1), in the regulation of CD4+ T cells and HBV vaccine responses during HCV infection. We demonstrated that KLRG1 was overexpressed on CD4+ T cells from HCV-infected, HBV vaccine nonresponders compared with HBV vaccine responders. The capacity of CD4+ T cells to proliferate and secrete IL-2 cytokine was inversely associated with the level of KLRG1 expression. Importantly, blocking KLRG1 signaling resulted in a significant improvement in CD4+ T cell proliferation and IL-2 production in HCV-infected, HBV vaccine nonresponders in response to TCR stimulation. Moreover, blockade of KLRG1 increased the phosphorylation of Akt (Ser473) and decreased the expression of cell cycle inhibitors p16ink4a and p27kip1, which subsequently enhanced the expression of cyclin-dependent kinase 2 and cyclin E. These results suggest that the KLRG1 pathway impairs CD4+ T cell responses to neoantigen and induces a state of immune senescence in individuals with HCV infection, raising the possibility that blocking this negative-signaling pathway might improve HBV vaccine responses in the setting of chronic viral infection. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|