Highly sensitive photoelectrochemical biosensor for kinase activity detection and inhibition based on the surface defect recognition and multiple signal amplification of metal-organic frameworks
| Autor: | Jiakun Su, Zonghua Wang, Lei Guo, Feng Wang, Zhiyong Yan, Jibao Cai, Yang Liu |
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| Rok vydání: | 2017 |
| Předmět: |
Biomedical Engineering
Biophysics Analytical chemistry Biosensing Techniques 02 engineering and technology 010402 general chemistry 01 natural sciences Ruthenium Limit of Detection Electrochemistry Animals Humans Chelation Kinase activity Protein kinase A Electrodes Metal-Organic Frameworks Enzyme Assays Photocurrent Detection limit Chemistry Kinase Electrochemical Techniques Equipment Design General Medicine 021001 nanoscience & nanotechnology Cyclic AMP-Dependent Protein Kinases Combinatorial chemistry 0104 chemical sciences MCF-7 Cells Cattle Metal-organic framework Zirconium 0210 nano-technology Oligopeptides Biosensor Biotechnology |
| Zdroj: | Biosensors and Bioelectronics. 97:107-114 |
| ISSN: | 0956-5663 |
| DOI: | 10.1016/j.bios.2017.05.011 |
| Popis: | A turn-on photoelectrochemical (PEC) biosensor based on the surface defect recognition and multiple signal amplification of metal-organic frameworks (MOFs) was proposed for highly sensitive protein kinase activity analysis and inhibitor evaluation. In this strategy, based on the phosphorylation reaction in the presence of protein kinase A (PKA), the Zr-based metal-organic frameworks (UiO-66) accommodated with [Ru(bpy)3]2+ photoactive dyes in the pores were linked to the phosphorylated kemptide modified TiO2/ITO electrode through the chelation between the Zr4+ defects on the surface of UiO-66 and the phosphate groups in kemptide. Under visible light irradiation, the excited electrons from [Ru(bpy)3]2+ adsorbed in the pores of UiO-66 injected into the TiO2 conduction band to generate photocurrent, which could be utilized for protein kinase activities detection. The large surface area and high porosities of UiO-66 facilitated a large number of [Ru(bpy)3]2+ that increased the photocurrent significantly, and afforded a highly sensitive PEC analysis of kinase activity. The detection limit of the as-proposed PEC biosensor was 0.0049UmL-1 (S/N!=!3). The biosensor was also applied for quantitative kinase inhibitor evaluation and PKA activities detection in MCF-7 cell lysates. The developed visible-light PEC biosensor provides a simple detection procedure and a cost-effective manner for PKA activity assays, and shows great potential in clinical diagnosis and drug discoveries. |
| Databáze: | OpenAIRE |
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