Differential accumulation of S-adenosylmethionine synthetase transcripts in response to salt stress
| Autor: | Joaquín Espartero, José M. Pardo, José Antonio Pintor-Toro |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 1994 |
| Předmět: |
Mutant
Molecular Sequence Data Salt stress Plant Science Biology Sodium Chloride Genes Plant Downregulation and upregulation Complementary DNA Vegetables Sequence Genetics medicine Mannitol Amino Acid Sequence RNA Messenger Lycopersicon esculentum Gene chemistry.chemical_classification Messenger RNA General Medicine Methionine Adenosyltransferase Blotting Northern Molecular biology Yeast Isoenzymes Blotting Southern SAM genes Enzyme chemistry Biochemistry Multigene Family DNA Probes Agronomy and Crop Science medicine.drug Abscisic Acid |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname |
| Popis: | NaCl stress causes the accumulation of several mRNAs in tomato seedlings. An upregulated cDNA clone, SAM1, was found to encode a S-adenosyl-L-methionine synthetase enzyme (AdoMet synthetase). Expression of the cDNA SAM1 in a yeast mutant lacking functional SAM genes resulted in high AdoMet synthetase activity and AdoMet accumulation. We show that tomato plants contain at least four SAM isogenes. Clones corresponding to isogenes SAM2 and SAM3 have also been isolated and sequenced. they encode predicted polypeptides 95% and 92% identical, respectively, to the SAM1-encoded AdoMet Synthetase. RNA hybridization analysis showed a differential response of SAM genes to salt and other stress treatments. SAM1 and SAM3 mRNAs accumulated in the root in response to NaCl, mannitol or ABA treatments. SAM1 mRNA accumulated also in leaf tissue. These increases of mRNA level were apparent as soon as 8 h after the initiation of the salt treatment and were maintained for at least 3 days. A possible role for AdoMet synthetases in the adaptation to salt stress is discussed. |
| Databáze: | OpenAIRE |
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