A novel gonad-specific Argonaute 4 serves as a defense against transposons in the black tiger shrimp Penaeus monodon
| Autor: | Apinunt Udomkit, Sakol Panyim, Suchitraporn Sukthaworn, Wantana Leebonoi |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
DNA
Complementary Molecular Sequence Data Piwi-interacting RNA Retrotransposon Roniviridae Aquatic Science Polymerase Chain Reaction Arthropod Proteins Penaeus monodon Penaeidae Animals Environmental Chemistry RasiRNA DNA transposon Amino Acid Sequence RNA Messenger Cloning Molecular Gonads Phylogeny RNA Double-Stranded RDE-1 Genetics Base Sequence biology fungi General Medicine Argonaute biology.organism_classification Shrimp Gene Expression Regulation Argonaute Proteins DNA Transposable Elements |
| Zdroj: | Fish & Shellfish Immunology. 42:280-288 |
| ISSN: | 1050-4648 |
| DOI: | 10.1016/j.fsi.2014.11.014 |
| Popis: | Argonaute is a key protein of the small-RNA guided gene regulation process. The Argonaute family is generally divided into two subfamilies; AGO and PIWI. In this study, a cDNA encoding a novel type of Argonaute (PmAgo4) in the black tiger shrimp Penaeus monodon was identified and characterized. PmAgo4 cDNA contained an open reading frame of 2433 nucleotides that can be translated into a deduced amino acid with the conserved PAZ and PIWI domains. PmAgo4 was phylogenetically clustered with the AGO subfamily while exhibited a gonad-specific expression pattern similar to that of proteins in the PIWI subfamily. The expression of PmAgo4 did not change significantly in response to either double-stranded RNA or yellow head virus injection suggesting that PmAgo4 may not be the main AGO proteins that play a role in dsRNA-mediated gene silencing or antiviral defense. Interestingly, PmAgo4 appeared to participate in the control of transposons since the activation of both DNA transposon and retrotransposon was detected in the testis of PmAgo4-knockdown shrimp. Our study thus provided the first evidence for an unusual type of the AGO proteins that was predominantly expressed in shrimp gonad and implication of its role in protecting the shrimp genome against an invasion of transposons. |
| Databáze: | OpenAIRE |
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