Cells on hierarchically-structured platforms hosting functionalized nanoparticles
| Autor: | Christina Lanara, Amelie Heuer-Jungemann, Emmanuel Stratakis, Costas Fotakis, Antonios G. Kanaras, C. Simitzi, Anthi Ranella, Aleka Manousaki, Pascal K. Harimech, Syrago Spanou |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
Silicon
Nanostructure Materials science Surface Properties Biomedical Engineering Metal Nanoparticles Nanotechnology Biocompatible Materials 02 engineering and technology 010402 general chemistry 01 natural sciences PC12 Cells Functionalized nanoparticles Animals General Materials Science Metal nanoparticles Nanoscopic scale Cell Proliferation RGD peptide Cell Differentiation 021001 nanoscience & nanotechnology Biocompatible material Small molecule 0104 chemical sciences Nanostructures Rats Colloidal gold Gold 0210 nano-technology Oligopeptides |
| Zdroj: | Biomaterials Science |
| Popis: | In this work, we report on a novel approach to develop hierarchically-structured cell culture platforms incorporating functionalized gold nanoparticles (AuNPs). In particular, the hierarchical substrates comprise primary pseudo-periodic arrays of silicon microcones combined with a secondary nanoscale pattern of homogenously deposited AuNPs terminated with bio-functional moieties. AuNPs with various functionalities (i.e. oligopeptides, small molecules and oligomers) were successfully attached onto the microstructures. Experiments with PC12 cells on the hierarchical substrates incorporating AuNPs carrying the RGD peptide showed an impressive growth and NGF-induced differentiation of the PC12 cells, compared to that on the NPs-free, bare, micropatterned substrates. The exploitation of the developed methodology for the binding of AuNPs as carriers of specific bio-functional moieties onto micropatterned culture substrates for cell biology studies is envisaged. |
| Databáze: | OpenAIRE |
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