Comparative structure, proximal promoter elements, and chromosome location of the human eosinophil major basic protein genes
| Autor: | William B. Johnson, L.L. Haley, Roger L. Eddy, Douglas A. Plager, Gerald J. Gleich, David A. Loegering, Thomas B. Shows, Deborah A. Weiler |
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| Rok vydání: | 2001 |
| Předmět: |
DNA
Complementary Transcription Genetic Centromere Molecular Sequence Data Biology Homology (biology) Eosinophil Major Basic Protein Conserved sequence Evolution Molecular Exon Mice Ribonucleases Sequence Homology Nucleic Acid Gene duplication Genetics Animals Humans Promoter Regions Genetic Gene Conserved Sequence In Situ Hybridization Fluorescence Gene Library Binding Sites Base Sequence Models Genetic Chromosomes Human Pair 11 Intron Chromosome Mapping Promoter Blood Proteins Exons Sequence Analysis DNA Eosinophil Granule Proteins Introns Major basic protein biology.protein CCAAT-Enhancer-Binding Proteins |
| Zdroj: | Genomics. 71(3) |
| ISSN: | 0888-7543 |
| Popis: | Human eosinophil major basic protein (MBP) is strongly implicated as a mediator of disease, especially bronchial asthma. We recently isolated a highly divergent human homologue of MBP (MBPH). Given human MBP's importance in disease and the restricted expression of it and human MBPH, we isolated the 4.6-kb human MBPH gene (HGMW-approved symbol PRG3). Comparisons among the human MBP (PRG2), human MBPH, and murine MBP-1 (mMBP-1; Prg2) genes suggest that the human MBP and mMBP-1 genes are more closely related than either is to the human MBPH gene. Proximal promoters of these three genes show conservation of potential binding sites for IK2 and STAT and of a known GATA site. However, a known C/EBP site is altered in the human MBPH gene's proximal promoter. The human MBP and MBPH genes localized to chromosome 11 in the centromere to 11q12 region. Thus, the human MBP and MBPH genes have diverged considerably, probably following a gene duplication event. Furthermore, the identified conserved and distinct proximal promoter elements likely contribute to the eosinophil-restricted and relatively reduced transcription of the human MBPH gene. |
| Databáze: | OpenAIRE |
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