Roles of TGF-β signaling pathway in endoplasmic reticulum stress in endothelial cells stimulated with cigarette smoke extract
| Autor: | Huifen Zhu, Qiu-li Ding, Hong Huang, Daofeng Yang |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
MAPK/ERK pathway Endoplasmic Reticulum Biochemistry Umbilical vein Flow cytometry 03 medical and health sciences Transforming Growth Factor beta Smoke Tobacco parasitic diseases Human Umbilical Vein Endothelial Cells Genetics medicine Humans Endoplasmic Reticulum Chaperone BiP Heat-Shock Proteins Messenger RNA medicine.diagnostic_test biology Chemistry Endoplasmic reticulum Interleukin-8 Endothelial Cells Transforming growth factor beta Endoplasmic Reticulum Stress Molecular biology 030104 developmental biology Gene Expression Regulation Unfolded protein response biology.protein Signal transduction Signal Transduction |
| Zdroj: | Current Medical Science. 37:699-704 |
| ISSN: | 2523-899X 2096-5230 |
| DOI: | 10.1007/s11596-017-1791-z |
| Popis: | To investigate the role of signaling pathway in the effect of endoplasmic reticulum stress (ER stress) in endothelial cells stimulated with cigarette smoke extract (CSE). Human umbilical vein endothelial cells (HUVECs) were cultured and divided into 3 groups: CSE-stimulated group, CSE-stimulated with 4-PBA group, and negative control group. HUVECs were cultured and stimulated with CSE at concentrations of 5%, 10% and 20%, respectively, mRNA of CXCL-8 and GRP78 was detected by real-time PCR. ELISA was performed to test the expression of CXCL-8 protein, and neutrophils migration was detected by Transwell board test. The NF-κB, ERK, p38MAPK and transforming growth factor beta (TGF-β) were detected by flow cytometry. The mRNA of CXCL-8 and GRP78 increased in CSE-stimulated HUVECs (P |
| Databáze: | OpenAIRE |
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