Protective efficacy of Hla-MntC-SACOL0723 fusion protein adjuvanted in alum and MPL against Staphylococcus aureus sepsis infection in mice
| Autor: | Ebrahim Eftekhar, Parivash Davoodian, Mehdi Hassaniazad, Sobhan Faezi, Tayebeh Ghaedi, Mohammad Ali Einakian, Khadijeh Ahmadi, Ali Atash Abparvar |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Methicillin-Resistant Staphylococcus aureus
0301 basic medicine Staphylococcus aureus Recombinant Fusion Proteins medicine.medical_treatment Immunology Monophosphoryl Lipid A chemical and pharmacologic phenomena Kidney medicine.disease_cause complex mixtures Sepsis Mice 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Immune system HLA Antigens Animals Immunology and Allergy Medicine Alum adjuvant Mice Inbred BALB C business.industry Alum Interleukin-17 Staphylococcal Vaccines Staphylococcal Infections medicine.disease Up-Regulation Vaccination Lipid A 030104 developmental biology chemistry Immunoglobulin G Periplasmic Binding Proteins Alum Compounds Female Interleukin-4 business Adjuvant 030215 immunology |
| Zdroj: | Journal of Immunological Methods. 494:113055 |
| ISSN: | 0022-1759 |
| DOI: | 10.1016/j.jim.2021.113055 |
| Popis: | To develop a suitable and effective vaccine against Staphylococcus aureus (S. aureus), we selected the Hla-MntC-SACOL0723 (HMS) recombinant protein with two different formulations of alum and Monophosphoryl lipid A (MPL) adjuvants. In this study, we aimed to evaluate the potentials of alum and MPL adjuvants in stimulating the immune response of HMS vaccine candidate against S. aureus. To evaluate the type of induced immune response, anti-HMS total IgG, IgG1, IgG2a, and IFN-γ, IL-2, IL-4, and IL-17 cytokines were determined after vaccination of mice with HMS-alum, HMS-MPL candidates. Mice were challenged with Methicillin-resistant Staphylococcus aureus (MRSA) was isolated from pressure sores and evaluated for bacterial load in the kidney homogenates and survival rate. It was observed that total IgG and isotypes (IgG1 and IgG2a), IL-4, and IL-17 were significantly increased in the group that received HMS-alum vaccine compared with the group that received HMS-MPL formulation. On the other hand, the levels of IFN-γ and IL-2 cytokines in the group that received HMS-MPL were higher than the group that received HMS-alum formulation. Bacterial load in the mice who received HMS protein formulated with alum adjuvant was reduced more than the mice who received HMS protein formulated with MPL adjuvant. Histopathological analysis showed more pathological changes in kidney tissues of the group received of HMS-MPL compared with the HMS-alum formulation. The survival rate was equal in both groups of immunized with HMS-alum and HMS-MPL formulations. Finally, it could be concluded that both adjuvants of alum and MPL are suitable immune response enhancers to HMS vaccine candidate. |
| Databáze: | OpenAIRE |
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