Analysis of the Major Patterns of B Cell Gene Expression Changes in Response to Short-Term Stimulation with 33 Single Ligands
| Autor: | Sun Young Lee, Xiaocui Zhu, Keng-Mean Lin, Jong-Woo Kim, Dennis Mock, Melvin I. Simon, Mi Sook Chang, Brian Saunders, Robert C. Hsueh, Sangdun Choi, Shankar Subramaniam, Paul C. Sternweis, Eugene Ke, Yun Anna Cao, David A. Fruman, William E. Seaman, Angela Alexander, Zhen Yan, Jamie A. Lee, Joella Grossoehme, Rebecca Hart, Richard H. Scheuermann |
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| Rok vydání: | 2004 |
| Předmět: |
Lipopolysaccharides
Male Chemokine CD40 Ligand Immunology B-Lymphocyte Subsets Stimulation Biology Ligands Lymphocyte Activation Polymerase Chain Reaction Mice Gene expression medicine Animals Immunology and Allergy Protein phosphorylation Receptor Gene Cells Cultured B cell Cell Proliferation Oligonucleotide Array Sequence Analysis Gene Expression Profiling Molecular biology Antibodies Anti-Idiotypic Up-Regulation Mice Inbred C57BL medicine.anatomical_structure Gene Expression Regulation CpG site biology.protein CpG Islands Interleukin-4 Spleen |
| Zdroj: | The Journal of Immunology. 173:7141-7149 |
| ISSN: | 1550-6606 0022-1767 |
| DOI: | 10.4049/jimmunol.173.12.7141 |
| Popis: | We examined the major patterns of changes in gene expression in mouse splenic B cells in response to stimulation with 33 single ligands for 0.5, 1, 2, and 4 h. We found that ligands known to directly induce or costimulate proliferation, namely, anti-IgM (anti-Ig), anti-CD40 (CD40L), LPS, and, to a lesser extent, IL-4 and CpG-oligodeoxynucleotide (CpG), induced significant expression changes in a large number of genes. The remaining 28 single ligands produced changes in relatively few genes, even though they elicited measurable elevations in intracellular Ca2+ and cAMP concentration and/or protein phosphorylation, including cytokines, chemokines, and other ligands that interact with G protein-coupled receptors. A detailed comparison of gene expression responses to anti-Ig, CD40L, LPS, IL-4, and CpG indicates that while many genes had similar temporal patterns of change in expression in response to these ligands, subsets of genes showed unique expression patterns in response to IL-4, anti-Ig, and CD40L. |
| Databáze: | OpenAIRE |
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