Genotoxicity testing of a fenugreek extract
Autor: | Maria A. Cifone, G.L. Erexson, A.M. Flammang, L.F. Stankowski |
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Rok vydání: | 2004 |
Předmět: |
Drug
Blood Glucose Male Salmonella typhimurium No-observed-adverse-effect level media_common.quotation_subject Mice Inbred Strains Toxicology medicine.disease_cause Ingredient Mice Diabetes mellitus medicine Diabetes Mellitus Escherichia coli Animals Humans Hypoglycemic Agents media_common Active ingredient No-Observed-Adverse-Effect Level Micronucleus Tests Plants Medicinal Traditional medicine Dose-Response Relationship Drug business.industry Mutagenicity Tests Plant Extracts United States Food and Drug Administration General Medicine medicine.disease United States Biotechnology Trigonella Consumer Product Safety Micronucleus test Toxicity Seeds business Genotoxicity Food Science |
Zdroj: | Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association. 42(11) |
ISSN: | 0278-6915 |
Popis: | Fenugreek seeds have been used in traditional medicines as a remedy for diabetes. Rich in protein, fenugreek seeds contain the unique major free amino acid 4-hydroxyisoleucine (4-OH-Ile), which has been characterized as one of the active ingredients for blood glucose control. Current use of fenugreek in foodstuff has been limited to its role as a flavoring agent, and not as an ingredient to help mitigate the blood glucose response for people with diabetes. As part of a safety evaluation of novel ingredients for use in blood glucose control, the potential genotoxicity of a fenugreek seed extract (THL), containing a minimum of 40% 4-OH-ILE, was evaluated using the standard battery of tests (reverse mutation assay; mouse lymphoma forward mutation assay; mouse micronucleus assay) recommended by US Food and Drug Administration (FDA) for food ingredients. THL was determined not to be genotoxic under the conditions of the tested genetic toxicity battery. The negative assay results provide support that addition of THL to foodstuffs formulated for people with diabetes is expected to be safe. A wide safety margin is established, as anticipated doses are small compared to the doses administered in the assays. |
Databáze: | OpenAIRE |
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