Short-Term Pulmonary Toxicity Assessment of Pre- and Post-incinerated Organomodified Nanoclay in Mice
| Autor: | Donna C. Davidson, Yon Rojanasakul, Todd A. Stueckle, Dale W. Porter, Cerasela Zoica Dinu, Ray Derk, Liying W. Rojanasakul, Alixandra Wagner, Lori A. Battelli, Marlene S. Orandle, Konstantinos A. Sierros, Tiffany G. Kornberg, Rakesh Kumar Gupta, Sushant Agarwal, Sherri Friend |
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| Rok vydání: | 2018 |
| Předmět: |
Male
0301 basic medicine Systemic blood Surface Properties Pulmonary toxicity Inflammatory response General Physics and Astronomy Inflammation Incineration 010501 environmental sciences Pharmacology 01 natural sciences Article Mice 03 medical and health sciences medicine Animals General Materials Science Particle Size Lung Pre and post 0105 earth and related environmental sciences Granuloma Chemistry Pulmonary inflammation General Engineering Pneumonia Platelet Activation Silicon Dioxide Mice Inbred C57BL 030104 developmental biology medicine.anatomical_structure Toxicity Bentonite Nanoparticles medicine.symptom |
| Zdroj: | ACS Nano. 12:2292-2310 |
| ISSN: | 1936-086X 1936-0851 |
| DOI: | 10.1021/acsnano.7b07281 |
| Popis: | Organomodified nanoclays (ONCs) are increasingly used as filler materials to improve nanocomposite strength, wettability, flammability, and durability. However, pulmonary risks associated with exposure along their chemical lifecycle are unknown. This study's objective was to compare pre- and post-incinerated forms of uncoated and organomodified nanoclays for potential pulmonary inflammation, toxicity, and systemic blood response. Mice were exposed via aspiration to low (30 μg) and high (300 μg) doses of preincinerated uncoated montmorillonite nanoclay (CloisNa), ONC (Clois30B), their respective incinerated forms (I-CloisNa and I-Clois30B), and crystalline silica (CS). Lung and blood tissues were collected at days 1, 7, and 28 to compare toxicity and inflammation indices. Well-dispersed CloisNa caused a robust inflammatory response characterized by neutrophils, macrophages, and particle-laden granulomas. Alternatively, Clois30B, I-Clois30B, and CS high-dose exposures elicited a low grade, persistent inflammatory response. High-dose Clois30B exposure exhibited moderate increases in lung damage markers and a delayed macrophage recruitment cytokine signature peaking at day 7 followed by a fibrotic tissue signature at day 28, similar to CloisNa. I-CloisNa exhibited acute, transient inflammation with quick recovery. Conversely, high-dose I-Clois30B caused a weak initial inflammatory signal but showed comparable pro-inflammatory signaling to CS at day 28. The data demonstrate that ONC pulmonary toxicity and inflammatory potential relies on coating presence and incineration status in that coated and incinerated nanoclay exhibited less inflammation and granuloma formation than pristine montmorillonite. High doses of both pre- and post-incinerated ONC, with different surface morphologies, may harbor potential pulmonary health hazards over long-term occupational exposures. |
| Databáze: | OpenAIRE |
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