Bone-marrow derived hematopoietic stem/progenitor cells express multiple isoforms of NADPH oxidase and produce constitutively reactive oxygen species
| Autor: | Nazzareno Capitanio, Maria Ripoli, Domenico Boffoli, Annamaria D'Aprile, Rosella Scrima, Antonio Tabilio, Claudia Piccoli, Lucia Lecce |
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| Rok vydání: | 2007 |
| Předmět: |
Morpholines
Blotting Western Biophysics Antigens CD34 Bone Marrow Cells Biology Models Biological Biochemistry Gene Expression Regulation Enzymologic chemistry.chemical_compound Onium Compounds Glutathione Peroxidase GPX1 Humans Enzyme Inhibitors Progenitor cell Molecular Biology Glutathione Peroxidase Microscopy Confocal NADPH oxidase Reverse Transcriptase Polymerase Chain Reaction Superoxide Dismutase Acetophenones NADPH Oxidases NOX4 Hydrogen Peroxide Cell Biology Catalase Flow Cytometry Hematopoietic Stem Cells Immunohistochemistry Cell biology Isoenzymes Endothelial stem cell Haematopoiesis chemistry Chromones NOX1 Apocynin cardiovascular system biology.protein Reactive Oxygen Species Adult stem cell |
| Zdroj: | Biochemical and Biophysical Research Communications. 353:965-972 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/j.bbrc.2006.12.148 |
| Popis: | Consolidated evidence highlights the importance of redox signalling in poising the balance between self-renewal and differentiation in adult stem cells. The present study shows that human hematopoietic stem/progenitor cells (HSCs) constitutively generate low levels of hydrogen peroxide whose production is inhibited by DPI, apocynin, catalase, and LY294002 and scarcely stimulated by PMA. Moreover, it is shown that HSCs express at the mRNA and protein levels the catalytic subunits of NOX1, NOX2, and NOX4 isoforms of the NADPH oxidase family along with the complete battery of the regulatory subunits p22, p40, p47, p67, rac1, rac2, NOXO1, and NOXA1 as well as the splicing variant NOX2s and that the three NOX isoforms are largely co-expressed in the same HSC. These findings are interpreted in terms of a positive feed-back mechanism of NOXs activation enabling a fine tuning of the ROS level to be possibly used in redox-mediated signalling for growth and differentiation of HSCs. |
| Databáze: | OpenAIRE |
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