Xenopus maternal RNAs from a dorsal animal blastomere induce a secondary axis in host embryos
| Autor: | Sally A. Moody, Alexandra M. Hainski |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
Blastomeres
Cytoplasm Mesoderm Polarity in embryogenesis Dorsal Lip Xenopus Xenopus laevis Morphogenesis medicine Animals RNA Messenger Molecular Biology Embryonic Induction Genetics biology Embryogenesis Embryo Gastrula Blastomere biology.organism_classification Cell biology Gastrulation Phenotype medicine.anatomical_structure Female Developmental Biology |
| Zdroj: | Development. 116:347-355 |
| ISSN: | 1477-9129 0950-1991 |
| Popis: | The initial steps of dorsal axis formation are controlled by localized maternal determinants in Drosophila, and a similar process has been proposed in Xenopus. The present study demonstrates that there are axis-inducing RNA molecules located in a specific dorsal midline, animal blastomere (D1.1) of the 16-cell-stage embryo. This blastomere, although in the animal hemisphere at cleavage stages, populates most of the dorsal lip of the blastopore, the region of Spemann’s organizer, during gastrulation, and is the major progenitor for dorsal mesodermal tissues. Cytosol from this blastomere causes ventral cells to take a more dorsal fate. RNA from this blastomere induces a secondary axis when injected into ventral blastomeres and restores the dorsal axis in UV-irradiated embryos. In Xenopus, activin B, goosecoid and Xwnt-8 RNAs can ectopically induce a dorsal axis; however, none is a maternal transcript. Therefore, the D1.1 blastomere probably contains dorsal determinant(s) that are either maternal members of these gene families, or other presently unknown molecule(s). Regardless of the identity of the determinant(s), this study presents the first indication that Xenopus maternal RNAs in the dorsal animal hemisphere are able to organize the dorsal axis. |
| Databáze: | OpenAIRE |
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