Organotypic slice cultures of human gastric and esophagogastric junction cancer

Autor: Florian Lordick, Achim Aigner, Christian Eckmann, Christian Moebius, Guido Schumacher, Nikolaus Gaßler, Justus Koerfer, Christian Wittekind, Ingo Bechmann, Arved Weimann, Sonja Kallendrusch, Nikolas Schopow, Daniela Geister, Volker Wiechmann, Woubet T. Kassahun, Felicitas Merz, Christoph Kubick
Rok vydání: 2016
Předmět:
0301 basic medicine
Cancer Research
Pathology
medicine.medical_specialty
Esophageal Neoplasms
esophagogastric junction cancer
Biopsy
Drug resistance
Biology
Tissue Culture Techniques
03 medical and health sciences
Cytokeratin
Organ Culture Techniques
0302 clinical medicine
Stomach Neoplasms
Antineoplastic Combined Chemotherapy Protocols
medicine
Humans
Radiology
Nuclear Medicine and imaging
Precision Medicine
Chemosensitivity
Original Research
Cisplatin
organotypic slice cultures
medicine.diagnostic_test
gastric cancer
Clinical Cancer Research
Cancer
medicine.disease
Combined Modality Therapy
personalized treatment
030104 developmental biology
Oncology
Drug Resistance
Neoplasm
030220 oncology & carcinogenesis
Immunohistochemistry
Histopathology
Esophagogastric Junction
Ex vivo
medicine.drug
Zdroj: Cancer Medicine
ISSN: 2045-7634
Popis: Gastric and esophagogastric junction cancers are heterogeneous and aggressive tumors with an unpredictable response to cytotoxic treatment. New methods allowing for the analysis of drug resistance are needed. Here, we describe a novel technique by which human tumor specimens can be cultured ex vivo, preserving parts of the natural cancer microenvironment. Using a tissue chopper, fresh surgical tissue samples were cut in 400 μm slices and cultivated in 6‐well plates for up to 6 days. The slices were processed for routine histopathology and immunohistochemistry. Cytokeratin stains (CK8, AE1/3) were applied for determining tumor cellularity, Ki‐67 for proliferation, and cleaved caspase‐3 staining for apoptosis. The slices were analyzed under naive conditions and following 2–4 days in vitro exposure to 5‐FU and cisplatin. The slice culture technology allowed for a good preservation of tissue morphology and tumor cell integrity during the culture period. After chemotherapy exposure, a loss of tumor cellularity and an increase in apoptosis were observed. Drug sensitivity of the tumors could be assessed. Organotypic slice cultures of gastric and esophagogastric junction cancers were successfully established. Cytotoxic drug effects could be monitored. They may be used to examine mechanisms of drug resistance in human tissue and may provide a unique and powerful ex vivo platform for the prediction of treatment response.
Databáze: OpenAIRE
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