Phylogenetic comparison and splice site conservation of eukaryotic U1 snRNP-specific U1-70K gene family
| Autor: | Mo-Xian Chen, Jing-Fang Yang, Yuan Tian, Qin-Lai Liu, Jianhua Zhang, Yinggao Liu, Yu-Zhen Zhao, Chen Chen, Shaoming Zhou, Tao Fan, Das Debatosh |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
0301 basic medicine Spliceosome Evolution In silico Science Computational biology Biology 01 natural sciences Article Ribonucleoprotein U1 Small Nuclear 03 medical and health sciences Splicing factor Protein Domains Genetics Animals Humans Gene family snRNP Amino Acid Sequence RNA Messenger Gene Phylogeny Multidisciplinary Sequence Homology Amino Acid Gene Expression Profiling Eukaryota 030104 developmental biology RNA splicing Medicine RNA Splicing Factors Precursor mRNA Protein Binding 010606 plant biology & botany |
| Zdroj: | Scientific Reports, Vol 11, Iss 1, Pp 1-15 (2021) Scientific Reports |
| ISSN: | 2045-2322 |
| Popis: | Eukaryotic cells can expand their coding ability by using their splicing machinery, spliceosome, to process precursor mRNA (pre-mRNA) into mature messenger RNA. The mega-macromolecular spliceosome contains multiple subcomplexes, referred to as small nuclear ribonucleoproteins (snRNPs). Among these, U1 snRNP and its central component, U1-70K, are crucial for splice site recognition during early spliceosome assembly. The human U1-70K has been linked to several types of human autoimmune and neurodegenerative diseases. However, its phylogenetic relationship has been seldom reported. To this end, we carried out a systemic analysis of 95 animal U1-70K genes and compare these proteins to their yeast and plant counterparts. Analysis of their gene and protein structures, expression patterns and splicing conservation suggest that animal U1-70Ks are conserved in their molecular function, and may play essential role in cancers and juvenile development. In particular, animal U1-70Ks display unique characteristics of single copy number and a splicing isoform with truncated C-terminal, suggesting the specific role of these U1-70Ks in animal kingdom. In summary, our results provide phylogenetic overview of U1-70K gene family in vertebrates. In silico analyses conducted in this work will act as a reference for future functional studies of this crucial U1 splicing factor in animal kingdom. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|