The effects of hyperthermia on human hepatocellular carcinoma stem and mature cancer cells
Autor: | Gerald Y. Minuk, David A. Miles, Jiaqi Yang, Julia Uhanova, Micah Grubert Van Iderstine, David P. Sontag |
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Rok vydání: | 2020 |
Předmět: |
Hyperthermia
Carcinoma Hepatocellular Hot Temperature Hepatocellular carcinoma Cell Survival Cell Population Specialties of internal medicine 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Recurrence Cancer stem cell Heat shock protein Humans Medicine HSP70 Heat-Shock Proteins HSP90 Heat-Shock Proteins RNA Messenger education Radiofrequency Ablation education.field_of_study Hepatology Cancer stem cells business.industry Liver Neoplasms Epithelial cell adhesion molecule Hep G2 Cells General Medicine medicine.disease medicine.anatomical_structure RC581-951 chemistry 030220 oncology & carcinogenesis Cancer cell Neoplastic Stem Cells Cancer research 030211 gastroenterology & hepatology Laser Therapy Neoplasm Recurrence Local business |
Zdroj: | Annals of Hepatology, Vol 19, Iss 3, Pp 265-268 (2020) |
ISSN: | 1665-2681 |
Popis: | Introduction and objectives Hepatocellular carcinoma (HCC) can recur following radiofrequency ablation and other hyperthermic treatment modalities. Cancer stem cells (CSCs) are a subpopulation of HCC cells that are difficult to eradicate and largely responsible for tumor recurrences. Thus, the principal objective of this study was to determine whether human HCC CSCs are relatively thermal-resistant compared to non-stem or mature cancer cells (MCCs). Materials and methods Epithelial cell adhesion molecule (EpCAM) positive enriched CSCs and EpCAM− MCCs were derived from a human HCC cell line using fluorescence activated cell sorting. Each cell population was exposed to 65 °C heat for 0–16 min and survival documented at various time points. Results Cell survival curves were similar in CSC and MCCs throughout the 16 min heat exposure period. Maximum killing was obtained after 12–14 min of heat exposure. Cytoprotective, heat shock proteins-70 (HSP70) and -90 (HSP90) mRNA expression were not disproportionately increased in CSCs. Conclusions These results suggest that human HCC CSCs are not more thermal resistant than MCCs and therefore, do not support the hypothesis that HCC recurrences following hyperthermic treatment reflect CSC thermal-resistance. |
Databáze: | OpenAIRE |
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