Behavioral changes occur earlier than redox alterations in developing zebrafish exposed to Mancozeb
| Autor: | Mauro Eugênio Medina Nunes, Illana Kemmerich Martins, Jeferson Luis Franco, Renata Siqueira de Mello, Luana Paganotto Leandro, Andressa Rubin Lopes, Dennis Guilherme da Costa Silva, Thaís Posser |
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| Rok vydání: | 2021 |
| Předmět: |
Embryo
Nonmammalian Antioxidant 010504 meteorology & atmospheric sciences Health Toxicology and Mutagenesis medicine.medical_treatment Population Physiology 010501 environmental sciences Toxicology medicine.disease_cause 01 natural sciences chemistry.chemical_compound Biomonitoring medicine Animals Mancozeb education Zebrafish 0105 earth and related environmental sciences Zineb education.field_of_study biology Embryo General Medicine biology.organism_classification Pollution Acetylcholinesterase Oxidative Stress chemistry Maneb Oxidation-Reduction Oxidative stress |
| Zdroj: | Environmental Pollution. 268:115783 |
| ISSN: | 0269-7491 |
| DOI: | 10.1016/j.envpol.2020.115783 |
| Popis: | As agriculture expands to provide food and wellbeing to the world’s growing population, there is a simultaneous increasing concern about the use of agrochemicals, which can harm non-target organisms, mainly in the aquatic environment. The fungicide Mancozeb (MZ) has been used on a large-scale and is a potent inducer of oxidative stress. Therefore, there is an urgent need for the development of more sensitive biomarkers designed to earlier biomonitoring of this compound. Here we tested the hypothesis that behavioral changes induced by sublethal MZ concentrations would occur first as compared to biochemical oxidative stress markers. Embryos at 4 h post-fertilization (hpf) were exposed to Mancozeb at 5, 10 and 20 μg/L. Controls were kept in embryo water only. Behavioral and biochemical parameters were evaluated at 24, 28, 72, and 168 hpf after MZ exposure. The results showed that MZ significantly altered spontaneous movement, escape responses, swimming capacity, and exploratory behavior at all exposure times. However, changes in ROS steady-stead levels and the activity of antioxidant enzymes were observable only at 72 and 168 hpf. In conclusion, behavioral changes occurred earlier than biochemical alterations in zebrafish embryos exposed to MZ, highlighting the potential of behavioral biomarkers as sensitive tools for biomonitoring programs. |
| Databáze: | OpenAIRE |
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