Improved Sensitivity of Sputum Smear Microscopy after Processing Specimens with C18-Carboxypropylbetaine To Detect Acid-Fast Bacilli: a Study of United States-Bound Immigrants from Vietnam
| Autor: | Truong Xuan Lien, Kayla F. Laserson, Vo Thi Chi Mai, Nancy J. Binkin, Susan A. Maloney, D. T. C. Nhung, Charles D. Wells, Wilbur D. Jones, Martin S. Cetron, Nguyen An Trinh, N. T. N. Lan, C. G. Thornton, Nguyen Huu Phuoc, Nguyen Thi Ngoc Yen, D. Q. An |
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| Jazyk: | angličtina |
| Rok vydání: | 2005 |
| Předmět: |
Microbiology (medical)
Adult Bacilli Tuberculosis Sensitivity and Specificity Microbiology Smear microscopy Specimen Handling Mycobacterium tuberculosis fluids and secretions Medicine Humans Tuberculosis Pulmonary Microscopy biology C18-carboxypropylbetaine Staining and Labeling business.industry Sputum Mycobacteriology and Aerobic Actinomycetes Emigration and Immigration biology.organism_classification medicine.disease bacterial infections and mycoses United States Staining respiratory tract diseases Betaine Vietnam Benzophenoneidum Acid-fast medicine.symptom business |
| Popis: | The goal of this study was to evaluate the effect of the specimen-processing method that uses the detergent C 18 -carboxypropylbetaine (CB-18) on the sensitivity of acid-fast bacillus (AFB) staining. Vietnamese immigrants with abnormal chest radiographs provided up to three sputum specimens, which were examined for acid-fast bacilli by use of direct auramine and Ziehl-Neelsen staining. The remaining sputum was split; half was cultured, and the other half was incubated with CB-18 for 24 h, centrifuged, and examined for AFB by both staining methods. CB-18 processing improved the sensitivity of AFB staining by 20 to 30% (only differences in auramine sensitivity were statistically significant) but reduced specificity by ≈20% ( P < 0.05). These findings have direct utility for overseas migrant tuberculosis screening programs, for which maximizing test sensitivity is a major objective. |
| Databáze: | OpenAIRE |
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